BMRB Entry 17196

Title:
Solution structure of the putative copper-ion-binding protein from Bacillus anthracis str. Ames
Deposition date:
2010-09-16
Original release date:
2012-08-01
Authors:
Zhang, Yongbo; Winsor, James; Dubrovska, Ievgeniia; Anderson, Wayne; Radhakrishnan, Ishwar; CSGID, CSGID
Citation:

Citation: Zhang, Yongbo; Winsor, James; Dubrovska, Ievgeniia; Anderson, Wayne; Radhakrishnan, Ishwar; CSGID, CSGID. "To be published"  .

Assembly members:

Assembly members:
Cu-binding protein, polymer, 71 residues, 7583.525 Da.

Natural source:

Natural source:   Common Name: Anthrax   Taxonomy ID: 1392   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus anthracis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG7

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts73
1H chemical shifts483

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cu-binding protein1

Entities:

Entity 1, Cu-binding protein 71 residues - 7583.525 Da.

1   SERASNALAMETGLUGLNLEUTHRLEUGLN
2   VALGLUGLYMETSERCYSGLYHISCYSVAL
3   ASNALAILEGLUSERSERVALLYSGLULEU
4   ASNGLYVALGLUGLNVALLYSVALGLNLEU
5   ALAGLUGLYTHRVALGLUVALTHRILEASP
6   SERSERVALVALTHRLEULYSASPILEVAL
7   ALAVALILEGLUASPGLNGLYTYRASPVAL
8   GLN

Samples:

sample_1: Cu-binding protein, [U-13C; U-15N], 1.1 mM; potassium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 1 mM; EDTA 1 mM; Protease Inhibitors trace na; sodium azide 1 mM; H2O 90%; D2O 10%

sample_2: Cu-binding protein, [U-13C; U-15N], 0.9 mM; potassium phosphate 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 1 mM; EDTA 1 mM; Protease Inhibitors trace na; sodium azide 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.9; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ARIA, Linge, O'Donoghue and Nilges - refinement

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAL19353 BAR75482 BAR82899 GAE99237 GAO60688
EMBL CCW06190 CDN37228 CGF80291 CGG45637 CIZ75704
GB AAP10657 AAP27596 AAS42664 AAT32973 AAT55880
REF NP_833456 NP_846110 WP_000436970 WP_000436971 WP_000436972

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks