BMRB Entry 17235

Title:
Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target AnphA.01018.a
Deposition date:
2010-10-02
Original release date:
2010-10-20
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Hewitt, Stephan; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter. "Solution structure of a putative acyl carrier protein from Anaplasma phagocytophilum, the bacterium responsible for human granulocytic ehrlichiosis."  .

Assembly members:

Assembly members:
ACP18, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anaplasma phagocytophilum   Taxonomy ID: 948   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anaplasma phagocytophilum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Data sets:
Data typeCount
13C chemical shifts370
15N chemical shifts94
1H chemical shifts583

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACP181

Entities:

Entity 1, ACP18 88 residues - Formula weight is not available

1   PROGLYSERMETVALSERGLUGLUILELYS
2   ALAGLNVALMETGLUSERVALILEGLYCYS
3   LEULYSLEUASNASPGLUGLNLYSGLNILE
4   LEUSERGLYTHRTHRASNLEUALALYSASP
5   PHEASNLEUASPSERLEUASPPHEVALASP
6   LEUILEMETSERLEUGLUGLUARGPHESER
7   LEUGLUILESERASPGLUASPALAGLNLYS
8   LEUGLUTHRVALASPASPILECYSARGTYR
9   ILEALASERLYSSERSERASPALA

Samples:

sample_1: ACP18, [U-100% 13C; U-100% 15N], 1 – 2 mM; sodium chloride 100 ± 3 mM; TRIS 20 ± 1 mM; H2O 93%; D2O 7%

sample_2: ACP18, [U-98% 15N], 1 – 2 mM; sodium chloride 100 ± 3 mM; TRIS 20 ± 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
deuterium exchangesample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

FELIX v2007, Accelrys Software Inc. - processing

SPARKY v3.115, Goddard - data analysis, peak picking

PSVS v1.1, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB ABD44176 AGR78947 AGR80194 AGR81449 EOA61179
REF WP_044104281 WP_044105205

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks