BMRB Entry 17421

Title:
Solution structure of human protein C6orf130 in complex with ADP-ribose
Deposition date:
2011-01-22
Original release date:
2011-03-08
Authors:
Lytle, B.; Peterson, F.; Volkman, B.
Citation:

Citation: Lytle, B.; Peterson, F.; Volkman, B.. "Solution structure of human C6orf130, a putative macro domain."  To be published ., .-..

Assembly members:

Assembly members:
C6orf130, polymer, 151 residues, 16904.613 Da.
APR, non-polymer, 559.316 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: obtained from a vendor   Host organism: Escherichia coli   Vector: pQE30

Data sets:
Data typeCount
13C chemical shifts649
15N chemical shifts161
1H chemical shifts1044

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C6orf1301
2ADENOSINE-5-DIPHOSPHORIBOSE2

Entities:

Entity 1, C6orf130 151 residues - 16904.613 Da.

The N-terminal glycine is a cloning artifact.

1   GLYSERSERLEUASNGLUASPPROGLUGLY
2   SERARGILETHRTYRVALLYSGLYASPLEU
3   PHEALACYSPROLYSTHRASPSERLEUALA
4   HISCYSILESERGLUASPCYSARGMETGLY
5   ALAGLYILEALAVALLEUPHELYSLYSLYS
6   PHEGLYGLYVALGLNGLULEULEUASNGLN
7   GLNLYSLYSSERGLYGLUVALALAVALLEU
8   LYSARGASPGLYARGTYRILETYRTYRLEU
9   ILETHRLYSLYSARGALASERHISLYSPRO
10   THRTYRGLUASNLEUGLNLYSSERLEUGLU
11   ALAMETLYSSERHISCYSLEULYSASNGLY
12   VALTHRASPLEUSERMETPROARGILEGLY
13   CYSGLYLEUASPARGLEUGLNTRPGLUASN
14   VALSERALAMETILEGLUGLUVALPHEGLU
15   ALATHRASPILELYSILETHRVALTYRTHR
16   LEU

Entity 2, ADENOSINE-5-DIPHOSPHORIBOSE - C15 H23 N5 O14 P2 - 559.316 Da.

1   APR

Samples:

sample: C6orf130, [U-100% 13C; U-100% 15N], 0.75 mM; ADP-ribose 1.875 mM; BisTris 20 mM; NaCl 200 mM; DTT 2 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sampleisotropicsample_conditions_1
3D F1-13C-flitered/F3-13C-edited 1H-13C NOESY aliphaticsampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker Avance II 600 MHz

Related Database Links:

BMRB 10164 15593
PDB
DBJ BAF83628 BAG36161
GB AAH11709 AIC53444 AIC58012 EAX04010 EAX04011
REF NP_001181654 NP_659500 XP_001117219 XP_001117235 XP_001174030
SP Q9Y530
AlphaFold Q9Y530

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks