BMRB Entry 17531

Title:
Three-dimensional structure of the cyclotide Cter M
Deposition date:
2011-03-16
Original release date:
2011-05-27
Authors:
Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David
Citation:

Citation: Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David. "From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes."  Proc. Natl. Acad. Sci. U.S.A. 108, 10127-10132 (2011).
PubMed: 21593408

Assembly members:

Assembly members:
entity, polymer, 29 residues, 3084.605 Da.

Natural source:

Natural source:   Common Name: Clitoria ternatea   Taxonomy ID: 43366   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Clitoria ternatea

Experimental source:

Experimental source:   Production method: chemical synthesis   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GLPTCGETCTLGTCYVPDCS CSWPICMKN

Data sets:
Data typeCount
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1circular proteins in legumes1

Entities:

Entity 1, circular proteins in legumes 29 residues - 3084.605 Da.

1   GLYLEUPROTHRCYSGLYGLUTHRCYSTHR
2   LEUGLYTHRCYSTYRVALPROASPCYSSER
3   CYSSERTRPPROILECYSMETLYSASN

Samples:

sample_1: Cter M 1 mM

sample_conditions_1: ionic strength: . M; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AEB92229 AEK26404 ALL96779
SP P86899
AlphaFold P86899