BMRB Entry 17584

Title:
1H 13C 15N resonance assignment of CD4+ T cell-derived peptide Thp5
Deposition date:
2011-04-11
Original release date:
2011-12-02
Authors:
PANDEY, NISHANT; BHAVESH, NEEL; DAS, GOBARDHAN
Citation:

Citation: Khan, Mohd Moin; Chatterjee, Samit; Dwivedi, Ved Prakash; Pandey, Nishant Kumar; Singh, Yogesh; Tousif, Sultan; Bhavesh, Neel Sarovar; Van Kaer, Luc; Das, Jyoti; Das, Gobardhan. "CD4+ T Cell-derived Novel Peptide Thp5 Induces Interleukin-4 Production in CD4+ T Cells to Direct T Helper 2 Cell Differentiation."  J. Biol. Chem. 287, 2830-2835 (2012).
PubMed: 22130674

Assembly members:

Assembly members:
Thp5, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Thp5: WRPYLQTEYYDVMTVISPPE FG

Data sets:
Data typeCount
13C chemical shifts58
15N chemical shifts19
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Thp51

Entities:

Entity 1, Thp5 22 residues - Formula weight is not available

1   TRPARGPROTYRLEUGLNTHRGLUTYRTYR
2   ASPVALMETTHRVALILESERPROPROGLU
3   PHEGLY

Samples:

sample_1: Thp5 800 uM; sodium chloride 150 mM; sodium phosphate 50 mM; D2O, [U-100% 2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
REF NP_081603

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks