BMRB Entry 17876

Title:
STRUCTURAL AND FUNCTIONAL ANALYSIS OF A NOVEL POTASSIUM TOXI ARGENTINEAN SCORPION TITYUS TRIVITTATUS REVEALS A NEW KAPPA SUB-FAMILY
Deposition date:
2011-08-19
Original release date:
2012-01-12
Authors:
Saucedo-Yanez, A.; Del Rio-Portilla, F.; Hernandez-Lopez, R.
Citation:

Citation: Saucedo, Alma Leticia; Flores-Solis, David; Rodriguez de la Vega, Ricardo; Ramirez-Cordero, Belen; Hernandez-Lopez, Rogelio; Cano-Sanchez, Patricia; Noriega-Navarro, Roxana; Garcia-Valdes, Jesus; Coronas-Valderrama, Fredy; de Roodt, Adolfo; Brieba, Luis; Possani, Lourival Domingos; Del Rio-Portilla, Federico. "New tricks of an old pattern: structural versatility of scorpion toxins with common cysteine spacing."  J. Biol. Chem. ., .-. (2012).
PubMed: 22238341

Assembly members:

Assembly members:
KAPPA-KTX3.1_SCORPION_TOXIN, polymer, 30 residues, 3334.915 Da.

Natural source:

Natural source:   Common Name: scorpions   Taxonomy ID: 369776   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Tityus trivittatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet32 modified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KAPPA-KTX3.1_SCORPION_TOXIN: GSGCMPEYCAGQCRGKVSQD YCLKNCRCIR

Data sets:
Data typeCount
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KAPPA-KTX3.1 SCORPION TOXIN1

Entities:

Entity 1, KAPPA-KTX3.1 SCORPION TOXIN 30 residues - 3334.915 Da.

1   GLYSERGLYCYSMETPROGLUTYRCYSALA
2   GLYGLNCYSARGGLYLYSVALSERGLNASP
3   TYRCYSLEULYSASNCYSARGCYSILEARG

Samples:

sample: KAPPA-KTX3.1 SCORPION TOXIN 2.3 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsampleisotropicsample_conditions_1
2D 1H-1H TOCSYsampleisotropicsample_conditions_1
2D DQF-Csampleisotropicsample_conditions_1

Software:

CYANA v2.1, D.A.CASE, ET AL. - refinement

NMRPipe, Delaglio, Zhengrong and Bax - structure solution

XEASY, Bartels et al. - structure solution

CARA v1.5, Keller and Wuthrich - structure solution

Molmol, Koradi, Billeter and Wuthrich - structure solution

AMBER, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • VARIAN INOVA 500 MHz
  • Varian UNITY 500 MHz

Related Database Links:

PDB
SP B3A0L5
AlphaFold B3A0L5