BMRB Entry 18107

Title:
The NMR structure of a major allergen from dust mite
Deposition date:
2011-11-29
Original release date:
2012-08-21
Authors:
Tan, Kang Wei; Mok, Yu Keung
Citation:

Citation: Tan, Kang Wei; Ong, Tan Ching; Gao, Yun Feng; Tiong, Yuen Sung; Wong, Kang Ning; Chew, Fook Tim; Mok, Yu Keung. "NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis."  J. Biol. Chem. 287, 34776-34785 (2012).
PubMed: 22887997

Assembly members:

Assembly members:
entity, polymer, 96 residues, 11208.752 Da.

Natural source:

Natural source:   Common Name: Blomia tropicalis   Taxonomy ID: 40697   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Blomia tropicalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Blomia tropicalis   Vector: pGEX-4T-1

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts94
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a major allergen from dust mite1

Entities:

Entity 1, a major allergen from dust mite 96 residues - 11208.752 Da.

1   ASNTHRALATHRGLNARGPHEHISGLUILE
2   GLULYSPHELEULEUHISILETHRHISGLU
3   VALASPASPLEUGLULYSTHRGLYASNLYS
4   ASPGLULYSALAARGLEULEUARGGLULEU
5   THRVALSERGLUALAPHEILEGLUGLYSER
6   ARGGLYTYRPHEGLNARGGLULEULYSARG
7   THRASPLEUASPLEULEUGLULYSPHEASN
8   PHEGLUALAALALEUALATHRGLYASPLEU
9   LEULEULYSASPLEULYSALALEUGLNLYS
10   ARGVALGLNASPSERGLU

Samples:

sample_1: sodium phosphate 0.8 mM; H2O 95%; D2O 5%; entity 0.8 ug/mL

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAX34047 ABH06344 ABH06345 ABH06346 ABH06347

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks