BMRB Entry 18701

Title:
Solution struture of cofilin like UNC-60B protein from Caenorhabditis elegans
Deposition date:
2012-09-04
Original release date:
2013-10-08
Authors:
SHUKLA, VAIBHAV KUMAR; YADAV, RAHUL; KABRA, ASHISH; JAIN, ANUPAM; KUMAR, DINESH; ONO, SHOICHIRO; ARORA, ASHISH
Citation:

Citation: SHUKLA, VAIBHAV KUMAR; YADAV, RAHUL; KABRA, ASHISH; JAIN, ANUPAM; KUMAR, DINESH; ONO, SHOICHIRO; ARORA, ASHISH. "Solution structure and dynamics of UNC-60B from Caenorhabditis elegans"  Biophys. J. ., .-..

Assembly members:

Assembly members:
entity, polymer, 152 residues, 17070.590 Da.

Natural source:

Natural source:   Common Name: Rhabditis elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETNH6

Data sets:
Data typeCount
13C chemical shifts646
15N chemical shifts144
1H chemical shifts1005

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UNC-60B from Caenorhabditis elegans1

Entities:

Entity 1, UNC-60B from Caenorhabditis elegans 152 residues - 17070.590 Da.

1   METALASERGLYVALLYSVALASPPROSER
2   CYSLYSASNALATYRASPLEULEUHISASN
3   LYSHISGLNHISSERTYRILEILEPHELYS
4   ILEASPLYSASNASPTHRALAILEVALVAL
5   GLULYSVALGLYGLULYSASNALAPROTYR
6   ALAGLUPHEVALGLUGLUMETLYSLYSLEU
7   VALGLUASPGLYLYSGLUCYSARGTYRALA
8   ALAVALASPVALGLUVALTHRVALGLNARG
9   GLNGLYALAGLUGLYTHRSERTHRLEUASN
10   LYSVALILEPHEVALGLNTYRCYSPROASP
11   ASNALAPROVALARGARGARGMETLEUTYR
12   ALASERSERVALARGALALEULYSALASER
13   LEUGLYLEUGLUSERLEUPHEGLNVALGLN
14   ALASERGLUMETSERASPLEUASPGLULYS
15   SERVALLYSSERASPLEUMETSERASNGLN
16   ARGILE

Samples:

sample_1: UNC-60B, [U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; H2O 93%; D2O 7%

sample_2: UNC-60B, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; H2O 93%; D2O 7%

sample_3: UNC-60B, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCO-NOESYsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
CB(CGCD)HDsample_2isotropicsample_conditions_1
CB(CGCDCE)HEsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - STRUCTURE CALCULATION

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS, Bhattacharya and Montelione - structure validation

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CCD67023
GB AAC14457 EGT30762
REF NP_503427 XP_002647499
SP Q07749
AlphaFold Q07749

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks