BMRB Entry 18712

Title:
b-flap domain of RNA polymerase (B. subtilis)
Deposition date:
2012-09-12
Original release date:
2014-03-10
Authors:
Mobli, Mehdi
Citation:

Citation: Mobli, Mehdi; Yang, Xiao; Ma, Cong; Thomas, Rhiannon; King, Glenn; Lewis, Peter. "NusA binding to the b-flap tip of RNA polymerase determined by NMR."  .

Assembly members:

Assembly members:
b-flap, polymer, 149 residues, 15446.424 Da.

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts409
15N chemical shifts138
1H chemical shifts902

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1b-flap domain of RNA polymerase (B. subtilis)1

Entities:

Entity 1, b-flap domain of RNA polymerase (B. subtilis) 149 residues - 15446.424 Da.

1   METASPASPVALTYRTHRSERILEHISILE
2   GLUGLUTYRGLUSERGLUALAARGASPTHR
3   LYSLEUGLYPROGLUGLUILETHRARGASP
4   ILEPROASNVALGLYGLUASPALALEUARG
5   ASNLEUASPASPARGGLYVALILEARGILE
6   GLYALAGLUVALLYSASPGLYASPLEULEU
7   VALGLYLYSVALTHRPROLYSGLYVALTHR
8   GLULEUTHRALAGLUGLUARGLEULEUHIS
9   ALAILEPHEGLYGLULYSALAARGGLUVAL
10   ARGASPTHRSERLEUARGVALPROHISGLY
11   GLYGLYGLYILEILEHISASPVALLYSVAL
12   PHEASNARGGLUASPGLYASPGLULEUPRO
13   PROGLYVALASNGLNLEUVALARGVALTYR
14   ILEVALGLNLYSARGLYSILESERGLUGLY
15   ASPGLYTHRHISHISHISHISHISHIS

Samples:

sample_1: b-flap, [U-100% 13C; U-100% 15N], 500 ± 50 uM; potassium phosphate 20 mM; sodium chloride 150 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

RNMRTK v3, Jeff Hoch, Alan Stern - processing

TALOS v+, Cornilescu, Delaglio and Bax - Dihedral angle generation

TOPSPIN v3, Bruker Biospin - collection

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
GB AFQ00666 AFQ00667 AGO57771 AHZ90577 AHZ90582

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks