BMRB Entry 19443

Title:
1H, 13C and 15N Backbone Assignment of the EC-1 Domain of Human E-Cadherin
Deposition date:
2013-08-20
Original release date:
2013-09-19
Authors:
Prasasty, Vivitri; Krause, Mary; Siahaan, Teruna; Laurence, Jennifer; Tambunan, Usman; Anbanandam, Asokan
Citation:

Citation: Prasasty, Vivitri; Krause, Mary; Tambunan, Usman; Anbanandam, Asokan; Laurence, Jennifer; Siahaan, Teruna. "(1)H, (13)C and (15)N backbone assignment of the EC-1 domain of human E-cadherin."  Biomol. NMR Assignments 9, 31-35 (2015).
PubMed: 24510398

Assembly members:

Assembly members:
EC1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pASK-IBA6

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts117
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EC11

Entities:

Entity 1, EC1 138 residues - Formula weight is not available

1   ASPTRPVALILEPROPROILESERCYSPRO
2   GLUASNGLULYSGLYPROPHEPROLYSASN
3   LEUVALGLNILELYSSERASNLYSASPLYS
4   GLUGLYLYSVALPHETYRSERILETHRGLY
5   GLNGLYALAASPTHRPROPROVALGLYVAL
6   PHEILEILEGLUARGGLUTHRGLYTRPLEU
7   LYSVALTHRGLUPROLEUASPARGGLUARG
8   ILEALATHRTYRTHRLEUPHESERHISALA
9   VALSERSERASNGLYASNALAVALGLUASP
10   PROMETGLUILELEUILETHRVALTHRASP
11   GLNASNASPASNLYSPROGLUPHETHRGLN
12   GLUVALPHELYSGLYSERVALMETGLUGLY
13   ALALEUPROGLYTHRSERVALMETGLUVAL
14   THRALATHRASPALAASPASPASP

Samples:

sample_1: potassium phosphate, KCl, CaCl2, DTT, [U-99% 13C; U-99% 15N], 1 ± 0.2 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks