BMRB Entry 19498

Title:
gp1.2
Deposition date:
2013-09-16
Original release date:
2016-04-18
Authors:
Mueller, Geoffrey; London, Robert; Schaaper, Roel
Citation:

Citation: Singh, Deepa. "The bacteriophage T7 encoded inhibitor (gp1.2) of E. coli dGTP triphosphohydrolase"  .

Assembly members:

Assembly members:
entity, polymer, 170 residues, 20389.061 Da.

Natural source:

Natural source:   Common Name: Bacteriophage T7   Taxonomy ID: 10760   Superkingdom: Viruses   Kingdom: not available   Genus/species: Bacteriophage T7

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDest566

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts75
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bacteriophage T7 encoded inhibitor (gp1.2)1

Entities:

Entity 1, bacteriophage T7 encoded inhibitor (gp1.2) 170 residues - 20389.061 Da.

1   METGLYARGLEUTYRSERGLYASNLEUALA
2   ALAPHELYSALAALATHRASNLYSLEUPHE
3   GLNLEUASPLEUALAVALILETYRASPASP
4   TRPTYRASPALATYRTHRARGLYSASPCYS
5   ILEARGLEUARGILEGLUASPARGSERGLY
6   ASNLEUILEASPTHRSERTHRPHETYRHIS
7   HISASPGLUASPVALLEUPHEASNMETCYS
8   THRASPTRPLEUASNHISMETTYRASPGLN
9   LEULYSASPTRPLYSMETGLYARGLEUTYR
10   SERGLYASNLEUALAALAPHELYSALAALA
11   THRASNLYSLEUPHEGLNLEUASPLEUALA
12   VALILETYRASPASPTRPTYRASPALATYR
13   THRARGLYSASPCYSILEARGLEUARGILE
14   GLUASPARGSERGLYASNLEUILEASPTHR
15   SERTHRPHETYRHISHISASPGLUASPVAL
16   LEUPHEASNMETCYSTHRASPTRPLEUASN
17   HISMETTYRASPGLNLEULYSASPTRPLYS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.2 mM; sodium chloride 46 mM; potassium chloride 1 mM; sodium phosphate 1.4 mM; potassium phosphate 0.05 mM; H2O 90%; D2O 10%

sample_1plusphage: entity, [U-100% 13C; U-100% 15N], 0.2 mM; sodium chloride 46 mM; potassium chloride 1 mM; sodium phosphate 1.4 mM; potassium phosphate 0.05 mM; Pf1 phage 50 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 69.8 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CS-Rosetta, Shen, Vernon, Baker and Bax - data analysis

CYANA, Herrmann, Guntert and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks