BMRB Entry 20017

Title:
NMR structure of Temporin-SHb in micellar SDS
Deposition date:
2008-04-09
Original release date:
2009-01-16
Authors:
Abbassi, Feten; Galanth, Cecile; Lequin, Olivier; Saito, Kazuko; Piesse, Christophe; Zargarian, Loussin; Hani, Khaled; Nicolas, Pierre; Amiche, Mohamed; Ladram, Ali
Citation:

Citation: Abbassi, Feten; Galanth, Cecile; Amiche, Mohamed; Saito, Kazuko; Piesse, Christophe; Zargarian, Loussine; Hani, Khaled; Nicolas, Pierre; Lequin, Olivier; Ladram, Ali. "Solution structure and model membrane interactions of temporins-SH, antimicrobial peptides from amphibian skin. A NMR spectroscopy and differential scanning calorimetry study"  Biochemistry 47, 10513-10525 (2008).
PubMed: 18795798

Assembly members:

Assembly members:
Temporin-SHb, polymer, 14 residues, 1399.732 Da.

Natural source:

Natural source:   Common Name: Rana saharica   Taxonomy ID: 70019   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana saharica

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Temporin-SHb: FLPIVTNLLSGLLX

Data sets:
Data typeCount
13C chemical shifts51
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Temporin-SHb1

Entities:

Entity 1, Temporin-SHb 14 residues - 1399.732 Da.

1   PHELEUPROILEVALTHRASNLEULEUSER
2   GLYLEULEUNH2

Samples:

sample_1: entity 1.5 mM; SDS, [U-99% 2H], 80 mM; DSS 0.1 mM

sample_conditions_1: pH: 5; temperature: 309.5 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

EMBL AM748900