BMRB Entry 21006

Title:
alpha-conotoxin ImI cystathionine 2-4
Deposition date:
2011-04-21
Original release date:
2011-10-27
Authors:
Dekan, Zoltan; Vetter, Irina; Daly, Norelle; Lewis, Richard; Alewood, Paul; Craik, David
Citation:

Citation: Dekan, Zoltan; Vetter, Irina; Daly, Norelle; Craik, David; Lewis, Richard; Alewood, Paul. "Alpha-Conotoxin ImI Incorporating Stable Cystathionine Bridges Maintains Full Potency and Identical Three-Dimensional Structure"  J. Am. Chem. Soc. 133, 15866-15869 (2011).
PubMed: 21899353

Assembly members:

Assembly members:
alpha_conotoxin_ImI_Cystathionine_2-4, polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha_conotoxin_ImI_Cystathionine_2-4: GCXSDPRCAWRC

Data sets:
Data typeCount
1H chemical shifts65

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha_conotoxin_ImI_Cystathionine_2-41

Entities:

Entity 1, alpha_conotoxin_ImI_Cystathionine_2-4 12 residues - Formula weight is not available

X=Cystathionine

1   GLYCYSCTTSERASPPROARGCYSALATRP
2   ARGCYS

Samples:

sample_1: alpha conotoxin ImI Cystathionine 2-4 1 mM; H2O 90%; D2O 10%

sample_conditions_1: temperature: 280 K; pH: 3

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz