BMRB Entry 25129

Title:
cBru9a
Deposition date:
2014-08-05
Original release date:
2015-07-20
Authors:
Akcan, Muharrem; Clark, Richard; Daly, Norelle; Conibear, Anne; de Faoite, Andrew; Heghinian, Mari; Adams, David; Mari, Frank; Craik, David
Citation:

Citation: Akcan, Muharrem; Clark, Richard; Daly, Norelle; Conibear, Anne; de Faoite, Andrew; Heghinian, Mari; Adams, David; Mari, Frank; Craik, David. "Transforming conotoxins into cyclotides: backbone cyclization of P-superfamily conotoxins"  Biopolymers ., .-. (2015).
PubMed: 26172377

Assembly members:

Assembly members:
cBru9a, polymer, 27 residues, 2809.185 Da.

Natural source:

Natural source:   Common Name: Wood's brown cone   Taxonomy ID: 101289   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus brunneus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cBru9a: SCGGSCFGGCWXGCSCYART CFRDGLP

Data sets:
Data typeCount
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cBru9a1

Entities:

Entity 1, cBru9a 27 residues - 2809.185 Da.

1   SERCYSGLYGLYSERCYSPHEGLYGLYCYS
2   TRPHYPGLYCYSSERCYSTYRALAARGTHR
3   CYSPHEARGASPGLYLEUPRO

Samples:

sample_1: cBru9a0.05 – 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB