BMRB Entry 25290

Title:
Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin in the E. coli Inner Membrane
Deposition date:
2014-10-17
Original release date:
2015-03-23
Authors:
Ward, Meaghan; Wang, Shenlin; Munro, Rachel; Ritz, Emily; Hung, Ivan; Gor'kov, Peter; Jiang, Yunjiang; Liang, Hongjun; Brown, Leonid; Ladizhansky, Vladimir
Citation:

Citation: Ward, Meaghan; Wang, Shenlin; Munro, Rachel; Ritz, Emily; Hung, Ivan; Gor'kov, Peter; Jiang, Yunjiang; Liang, Hongjun; Brown, Leonid; Ladizhansky, Vladimir. "In Situ Structural Studies of Anabaena Sensory Rhodopsin in the E. coli Membrane"  Biophys. J. 108, 1683-1696 (2015).

Assembly members:

Assembly members:
Anabaena_sensory_rhodopsin, polymer, 235 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anabaena (Nostoc)   Taxonomy ID: 103690   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena PCC7120

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKJ606

Data sets:
Data typeCount
13C chemical shifts167
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASR, chain 11
2ASR, chain 21
3ASR, chain 31

Entities:

Entity 1, ASR, chain 1 235 residues - Formula weight is not available

1   METASNLEUGLUSERLEULEUHISTRPILE
2   TYRVALALAGLYMETTHRILEGLYALALEU
3   HISPHETRPSERLEUSERARGASNPROARG
4   GLYVALPROGLNTYRGLUTYRLEUVALALA
5   METPHEILEPROILETRPSERGLYLEUALA
6   TYRMETALAMETALAILEASPGLNGLYLYS
7   VALGLUALAALAGLYGLNILEALAHISTYR
8   ALAARGTYRILEASPTRPMETVALTHRTHR
9   PROLEULEULEULEUSERLEUSERTRPTHR
10   ALAMETGLNPHEILELYSLYSASPTRPTHR
11   LEUILEGLYPHELEUMETSERTHRGLNILE
12   VALVALILETHRSERGLYLEUILEALAASP
13   LEUSERGLUARGASPTRPVALARGTYRLEU
14   TRPTYRILECYSGLYVALCYSALAPHELEU
15   ILEILELEUTRPGLYILETRPASNPROLEU
16   ARGALALYSTHRARGTHRGLNSERSERGLU
17   LEUALAASNLEUTYRASPLYSLEUVALTHR
18   TYRPHETHRVALLEUTRPILEGLYTYRPRO
19   ILEVALTRPILEILEGLYPROSERGLYPHE
20   GLYTRPILEASNGLNTHRILEASPTHRPHE
21   LEUPHECYSLEULEUPROPHEPHESERLYS
22   VALGLYPHESERPHELEUASPLEUHISGLY
23   LEUARGASNLEUASNASPSERARGGLNHIS
24   HISHISHISHISHIS

Samples:

IM-ASR: IM-ASR, [U-100% 13C; U-100% 15N], 0.053 mg/ul; E. coli inner membrane 0.93 mg/ul; H2O 100%

sample_conditions_1: pH: 9; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D NCAIM-ASRsolidsample_conditions_1
3D CANCOIM-ASRsolidsample_conditions_1
3D NCACBIM-ASRsolidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 17064 18595
PDB
DBJ BAB74864
REF WP_010997316