BMRB Entry 25570

Title:
Structure of the C-terminal membrane domain of HCV NS5B protein
Deposition date:
2015-04-15
Original release date:
2016-04-18
Authors:
Montserret, Roland; Penin, Francois
Citation:

Citation: Montserret, Roland; Penin, Francois. "Structure of the C-terminal membrane domain of HCV NS5B protein"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity, polymer, 30 residues, 3483.139 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 31646   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: HSVSHARPRWFWFSLLLLAA GVGIYLLPNR

Data sets:
Data typeCount
13C chemical shifts102
15N chemical shifts24
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ns5bc1

Entities:

Entity 1, ns5bc 30 residues - 3483.139 Da.

NS5B genotype 1a

1   HISSERVALSERHISALAARGPROARGTRP
2   PHETRPPHESERLEULEULEULEUALAALA
3   GLYVALGLYILETYRLEULEUPROASNARG

Samples:

sample_1: entity 1.4 ± 0.05 mM; SDS, [U-2H], 1 M; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

X-PLOR_NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 1000 MHz

Related Database Links:

GB AF009606
UNP P27958
AlphaFold O36610

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks