BMRB Entry 25742

Title:
TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy
Deposition date:
2015-08-03
Original release date:
2016-07-11
Authors:
Thurston, T.; Allen, Mark; Ravenhill, B.; Karpiyevitch, M.; Bloor, S.; Kaul, A.; Matthews, S.; Komander, D.; Holden, D.; Bycroft, M.; Randow, F.; Matthews, A.
Citation:

Citation: Thurston, Teresa Lm; Boyle, Keith; Allen, Mark; Ravenhill, Benjamin; Karpiyevich, Maryia; Bloor, Stuart; Kaul, Annie; Noad, Jessica; Foeglein, Agnes; Matthews, Sophie; Komander, David; Bycroft, Mark; Randow, Felix. "Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy"  EMBO J. 35, 1779-1792 (2016).
PubMed: 27370208

Assembly members:

Assembly members:
OPTINEURIN, polymer, 32 residues, 3514.0982 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: HLTV plasmid

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OPTINEURIN: GSRNIPIHSCPKCGEVLPDI DTLQIHVMDCII

Data sets:
Data typeCount
13C chemical shifts104
15N chemical shifts25
1H chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1OPTINEURIN1
2ZINC ION2

Entities:

Entity 1, OPTINEURIN 32 residues - 3514.0982 Da.

1   GLYSERARGASNILEPROILEHISSERCYS
2   PROLYSCYSGLYGLUVALLEUPROASPILE
3   ASPTHRLEUGLNILEHISVALMETASPCYS
4   ILEILE

Entity 2, ZINC ION - 65.409 Da.

1   ZN

Samples:

sample_1: OPTINEURIN, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%; Tris 10 mM; NaCl 135 mM; B-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
DQF-COSYsample_1solutionsample_conditions_1
HSQCsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCACONHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HNCACOsample_1solutionsample_conditions_1
HNHBsample_1solutionsample_conditions_1

Software:

Ansig vany, Kraulis - chemical shift assignment

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP OPTN_HUMAN
AlphaFold Q9Y218

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks