BMRB Entry 26775

Title:
Backbone and side chain 1H, 13C, and 15N Chemical Shift assignments for Plasmodium falciparum SUMO.
Deposition date:
2016-04-12
Original release date:
2016-10-14
Authors:
Singh, Jai Shankar; Gujrati, Mansi; Shukla, Vaibhav; Mishra, Ram; Kumar, Ashutosh
Citation:

Citation: Singh, Jai Shankar; Shukla, Vaibhav Kumar; Gujrati, Mansi; Mishra, Ram Kumar; Kumar, Ashutosh. "Backbone and side-chain resonance assignments of Plasmodium falciparum SUMO"  Biomol NMR Assign 11, 17-20 (2017).
PubMed: 27699617

Assembly members:

Assembly members:
Plasmodium_falciparum_SUMO, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts93
1H chemical shifts556

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PfSUMO1

Entities:

Entity 1, PfSUMO 98 residues - Formula weight is not available

1   METGLYASPASPASPSERALAVALASNASN
2   ASNGLYSERSERPROVALASNASNGLNGLY
3   GLUHISILEGLNVALLYSVALARGSERPRO
4   ASPGLYALAGLUVALPHEPHELYSILELYS
5   ARGLYSTHRLYSLEUGLULYSLEUMETGLU
6   VALTYRCYSASNARGLEUGLYGLNSERMET
7   GLUALAVALARGPHELEUTYRASPGLYASP
8   ARGILEHISGLYASPASNTHRPROGLUGLN
9   LEUGLYILEGLUASPGLYASPVALILEASP
10   ALAMETVALGLNGLNTHRGLYGLY

Samples:

PfSUMO_1: Plasmodium falciparum SUMO (PfSUMO), [U-15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; D2O 10%

PfSUMO_2: Plasmodium falciparum SUMO (PfSUMO), [U-13C; U-15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; D2O 10%

PfSUMO_3: Plasmodium falciparum SUMO (PfSUMO), [U-13C; U-15N], 1 mM; TRIS 20 mM; sodium chloride 50 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCPfSUMO_1isotropicsample_conditions_1
3D HNNPfSUMO_2isotropicsample_conditions_1
3D CBCA(CO)NHPfSUMO_2isotropicsample_conditions_1
3D CBCANHPfSUMO_2isotropicsample_conditions_1
3D HNCOPfSUMO_2isotropicsample_conditions_1
3D HN(CA)COPfSUMO_2isotropicsample_conditions_1
3D TOCSY-HSQCPfSUMO_1isotropicsample_conditions_1
3D HCC(CO)NHPfSUMO_2isotropicsample_conditions_1
3D CC(CO)NHPfSUMO_2isotropicsample_conditions_1
2D 1H-13C HSQCPfSUMO_2isotropicsample_conditions_1
3D 1H-15N NOESYPfSUMO_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticPfSUMO_3isotropicsample_conditions_1

Software:

CcpNmr v2.3.1, CCPN - chemical shift assignment

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

TOPSPIN v3.2, Bruker Biospin - processing

TALOS vtalos+, Cornilescu, Delaglio and Bax - Secodary Strucutre Prediction

NMR spectrometers:

  • Bruker Ascend 750 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz

Related Database Links:

NCBI XP_001351616.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks