BMRB Entry 26840

Title:
'1H, 13C, and 15N Chemical Shift Assignments for the periplasmic domain of GacS histidine-kinase'
Deposition date:
2016-06-27
Original release date:
2017-02-20
Authors:
Ali-Ahmad, Ahmad; Bornet, Olivier; Guerlesquin, Francoise; Sebban-Kreuzer, Corinne
Citation:

Citation: Ali Ahmad, Ahmad; Bornet, Olivier; Guerlesquin, Francoise; Bourne, Yves; Vincent, Florence; Sebban-Kreuzer, Corinne. "NMR assignments of the GacS histidine-kinase periplasmic detection domain from Pseudomonas aeruginosa PAO1"  Biomol. NMR Assignments 11, 25-28 (2017).
PubMed: 27714507

Assembly members:

Assembly members:
GacSperi, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts568
15N chemical shifts148
1H chemical shifts968

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GacSperi monomer1

Entities:

Entity 1, GacSperi monomer 150 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISSERSER
2   GLYVALASPLEUGLYTHRGLUASNLEUTYR
3   PHEGLNSERMETARGALAGLNLEUILEGLU
4   ARGGLYGLNLEUILEALAGLUGLNLEUALA
5   PROLEUALAALATHRALALEUALAARGLYS
6   ASPTHRALAVALLEUASNARGILEALAASN
7   GLUALALEUASPGLNPROASPVALARGALA
8   VALTHRPHELEUASPALAARGGLNGLUARG
9   LEUALAHISALAGLYPROSERMETLEUTHR
10   VALALAPROALAGLYASPALASERHISLEU
11   SERMETSERTHRGLULEUASPTHRTHRHIS
12   PHELEULEUPROVALLEUGLYARGHISHIS
13   SERLEUSERGLYALATHRGLUPROASPASP
14   GLUARGVALLEUGLYTRPVALGLULEUGLU
15   LEUSERHISHISGLYTHRLEULEUARGGLY

Samples:

sample_1: GacSperi 1 mM; sodium phosphate 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_2: GacSperi, [U-100% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_3: GacSperi, [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HCACOsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
2D CBCACOsample_3isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.9, CARA Definition Team (CDT) - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Uniform NMR System 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks