BMRB Entry 26862

Title:
Stress Response Peptide-2; Manduca Sexta
Deposition date:
2016-07-27
Original release date:
2017-02-16
Authors:
Schrag, Lynn; Cao, Xiaolong; Herrera, Alvaro; Wang, Yang; Jiang, Haobo; Prakash, Om
Citation:

Citation: Schrag, Lynn Goss; Cao, Xiaolong; Herrera, Alvaro Ivan; Wang, Yang; Jiang, Haobo; Prakash, Om. "Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2"  Protein Pept. Lett. 24, 3-11 (2017).
PubMed: 27903232

Assembly members:

Assembly members:
Stress_Response_Peptide-2, polymer, 25 residues, 2751.12 Da.

Natural source:

Natural source:   Common Name: tobacco hornworm   Taxonomy ID: 7130   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Manduca Sexta

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Stress_Response_Peptide-2: FGVKDGKCPSGRVRRLGICV PDDDY

Data sets:
Data typeCount
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Stress Response Peptide-21

Entities:

Entity 1, Stress Response Peptide-2 25 residues - 2751.12 Da.

1   PHEGLYVALLYSASPGLYLYSCYSPROSER
2   GLYARGVALARGARGLEUGLYILECYSVAL
3   PROASPASPASPTYR

Samples:

sample_1: Stress Response Peptide-2 2.2 mM

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

VNMRJ v3.2, Varian - collection

CCPNMR_Analysis v2.4, Vranken - chemical shift assignment, peak picking

SPARKY v3, Goddard - chemical shift assignment, peak picking, processing

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

  • Varian Oxford 500 MHz