BMRB Entry 26953

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Kruppel-like Factor 4 (KLF4) Residues 1-130
Deposition date:
2016-11-22
Original release date:
2016-11-23
Authors:
Conroy, Brigid; Langelaan, David; Smith, Steven
Citation:

Citation: Conroy, Brigid; Weiss, Emma; Smith, Steven; Langelaan, David. "Backbone 1H, 13C, and 15N NMR resonance assignments of the Kruppel-like factor 4 activation domain"  Biomol. NMR Assign. 11, 95-98 (2017).
PubMed: 28247282

Assembly members:

Assembly members:
KLF4_1-130, polymer, 132 residues, 13757.2305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts116
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KLF4 1-1301

Entities:

Entity 1, KLF4 1-130 132 residues - 13757.2305 Da.

A Gly-Ser cloning artifact has been added to the N-terminus of the KLF4 1-130 fragment.

1   GLYSERMETARGGLNPROPROGLYGLUSER
2   ASPMETALAVALSERASPALALEULEUPRO
3   SERPHESERTHRPHEALASERGLYPROALA
4   GLYARGGLULYSTHRLEUARGGLNALAGLY
5   ALAPROASNASNARGTRPARGGLUGLULEU
6   SERHISMETLYSARGLEUPROPROVALLEU
7   PROGLYARGPROTYRASPLEUALAALAALA
8   THRVALALATHRASPLEUGLUSERGLYGLY
9   ALAGLYALAALACYSGLYGLYSERASNLEU
10   ALAPROLEUPROARGARGGLUTHRGLUGLU
11   PHEASNASPLEULEUASPLEUASPPHEILE
12   LEUSERASNSERLEUTHRHISPROPROGLU
13   SERVALALAALATHRVALSERSERSERALA
14   SERALA

Samples:

sample_1: MES 20 mM; DTT 5 mM; KLF4 1-130, [U-100% 13C; U-100% 15N], 1.3 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

NMRView v8.0.3, Johnson, One Moon Scientific - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP O43474
AlphaFold Q9UNP3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks