BMRB Entry 26955

Title:
Fyn SH3 WT delta57
Deposition date:
2016-11-23
Original release date:
2017-02-06
Authors:
Libich, David; Tugarinov, Vitali; Ghirlando, Rodolfo; Clore, G. Marius
Citation:

Citation: Libich, David; Tugarinov, Vitali; Ghirlando, Rodolfo; Clore, G. Marius. "Confinement and Stabilization of Fyn SH3 Folding Intermediate Mimetics within the Cavity of the Chaperonin GroEL Demonstrated by Relaxation-Based NMR"  Biochemistry 56, 903-906 (2017).
PubMed: 28156097

Assembly members:

Assembly members:
WTd57, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts180
15N chemical shifts60
1H chemical shifts60
residual dipolar couplings52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WTd571

Entities:

Entity 1, WTd57 62 residues - Formula weight is not available

1   GLYALAMETVALGLNILESERTHRLEUPHE
2   GLUALALEUTYRASPTYRGLUALAARGTHR
3   GLUASPASPLEUSERPHEHISLYSGLYGLU
4   LYSPHEGLNILELEUASNSERSERGLUGLY
5   ASPTRPTRPGLUALAARGSERLEUTHRTHR
6   GLYGLUTHRGLYTYRILEPROSERASNTYR
7   VALALA

Samples:

sample_1: WTd57, [U-13C; U-15N], 0.6 mM; sodium phosphate 50 mM; EDTA 0.2 mM; sodium azide 0.05 % w/v

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks