BMRB Entry 27039

Title:
Chemical shifts of amorphous-looking aggregates of the P23T mutant of human gamma-D-crystallin formed at pH 7.
Deposition date:
2017-02-26
Original release date:
2017-05-02
Authors:
Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick
Citation:

Citation: Boatz, Jennifer; Whitley, Matthew; Li, Mingyue; Gronenborn, Angela; van der Wel, Patrick. "Cataract-associated P23T gamma-D-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH"  Nat Commun 8, 15137-15137 (2017).
PubMed: 28474685

Assembly members:

Assembly members:
gD_crystallin_P23T, polymer, 173 residues, 20616.9178 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14b

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gDcrystallinP23T1

Entities:

Entity 1, gDcrystallinP23T 173 residues - 20616.9178 Da.

1   GLYLYSILETHRLEUTYRGLUASPARGGLY
2   PHEGLNGLYARGHISTYRGLUCYSSERSER
3   ASPHISTHRASNLEUGLNPROTYRLEUSER
4   ARGCYSASNSERALAARGVALASPSERGLY
5   CYSTRPMETLEUTYRGLUGLNPROASNTYR
6   SERGLYLEUGLNTYRPHELEUARGARGGLY
7   ASPTYRALAASPHISGLNGLNTRPMETGLY
8   LEUSERASPSERVALARGSERCYSARGLEU
9   ILEPROHISSERGLYSERHISARGILEARG
10   LEUTYRGLUARGGLUASPTYRARGGLYGLN
11   METILEGLUPHETHRGLUASPCYSSERCYS
12   LEUGLNASPARGPHEARGPHEASNGLUILE
13   HISSERLEUASNVALLEUGLUGLYSERTRP
14   VALLEUTYRGLULEUSERASNTYRARGGLY
15   ARGGLNTYRLEULEUMETPROGLYASPTYR
16   ARGARGTYRGLNASPTRPGLYALATHRASN
17   ALAARGVALGLYSERLEUARGARGVALILE
18   ASPPHESER

Samples:

gDcrytallinP23T: gD crystallin P23T, [U-99% 13C; U-99% 15N], mg

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXgDcrytallinP23Tisotropicsample_conditions_1
3D NCOCXgDcrytallinP23Tisotropicsample_conditions_1
2D DARRgDcrytallinP23Tisotropicsample_conditions_1
2D NCACXgDcrytallinP23Tisotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P07320
AlphaFold Q99681