BMRB Entry 27061

Title:
Backbone assignments of Escherichia coli. holo-Acyl Carrier protein
Deposition date:
2017-03-30
Original release date:
2017-03-31
Authors:
Marcella, Aaron; Barb, Adam
Citation:

Citation: Marcella, Aaron; Barb, Adam. "Backbone assignment of E. coli acyl carrier protein"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
holo-Acyl_Carrier_Protein(ACPP), polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDUET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
holo-Acyl_Carrier_Protein(ACPP): MGSTIEERVKKIIGEQLGVK QEEVTNNASFVEDLGADSLD TVELVMALEEEFDTEIPDEE AEKITTVQAAIDYINGHQA

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ACPP1

Entities:

Entity 1, ACPP 79 residues - Formula weight is not available

1   METGLYSERTHRILEGLUGLUARGVALLYS
2   LYSILEILEGLYGLUGLNLEUGLYVALLYS
3   GLNGLUGLUVALTHRASNASNALASERPHE
4   VALGLUASPLEUGLYALAASPSERLEUASP
5   THRVALGLULEUVALMETALALEUGLUGLU
6   GLUPHEASPTHRGLUILEPROASPGLUGLU
7   ALAGLULYSILETHRTHRVALGLNALAALA
8   ILEASPTYRILEASNGLYHISGLNALA

Samples:

holo-Acyl_Carrier_Protein: D2O, [U-2H], 5%; DSS 1 mM; DTT 1 mM; H2O 95%; sodium chloride 100 mM; MgCl2 10 mM; MOPS 25 mM; holo-Acyl Carrier Protein(ACPP), [U-13C; U-15N], 1.2 mM

sample_conditions_1: ionic strength: 110 mM; pH: 7.1; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOholo-Acyl_Carrier_Proteinisotropicsample_conditions_1
3D CBCA(CO)NHholo-Acyl_Carrier_Proteinisotropicsample_conditions_1
3D CBCANHholo-Acyl_Carrier_Proteinisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks