BMRB Entry 4287

Title:
1H and 15N Chemical Shift Assignments of the Unstructured Yeast Target Membrane SNARE Sso1.
Deposition date:
1998-12-18
Original release date:
1999-10-05
Authors:
Fiebig, Klaus; Brunger, Axel
Citation:

Citation: Fiebig, Klaus; Rice, Luke; Pollock, Elizabeth; Brunger, Axel. "Folding Intermediates of SNARE Complex Assembly"  Nat. Struct. Biol. 6, 117-123 (1999).

Assembly members:

Assembly members:
Sso1, polymer, 268 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
15N chemical shifts76
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso11

Entities:

Entity 1, Sso1 268 residues - Formula weight is not available

1   GLYSERHISMETSERTYRASNASNPROTYR
2   GLNLEUGLUTHRPROPHEGLUGLUSERTYR
3   GLULEUASPGLUGLYSERSERALAILEGLY
4   ALAGLUGLYHISASPPHEVALGLYPHEMET
5   ASNLYSILESERGLNILEASNARGASPLEU
6   ASPLYSTYRASPHISTHRILEASNGLNVAL
7   ASPSERLEUHISLYSARGLEULEUTHRGLU
8   VALASNGLUGLUGLNALASERHISLEUARG
9   HISSERLEUASPASNPHEVALALAGLNALA
10   THRASPLEUGLNPHELYSLEULYSASNGLU
11   ILELYSSERALAGLNARGASPGLYILEHIS
12   ASPTHRASNLYSGLNALAGLNALAGLUASN
13   SERARGGLNARGPHELEULYSLEUILEGLN
14   ASPTYRARGILEVALASPSERASNTYRLYS
15   GLUGLUASNLYSGLUGLNALALYSARGGLN
16   TYRMETILEILEGLNPROGLUALATHRGLU
17   ASPGLUVALGLUALAALAILESERASPVAL
18   GLYGLYGLNGLNILEPHESERGLNALALEU
19   LEUASNALAASNARGARGGLYGLUALALYS
20   THRALALEUALAGLUVALGLNALAARGHIS
21   GLNGLULEULEULYSLEUGLULYSSERMET
22   ALAGLULEUTHRGLNLEUPHEASNASPMET
23   GLUGLULEUVALILEGLUGLNGLNGLUASN
24   VALASPVALILEASPLYSASNVALGLUASP
25   ALAGLNLEUASPVALGLUGLNGLYVALGLY
26   HISTHRASPLYSALAVALLYSSERALAARG
27   LYSALAARGLYSASNLYSILEARG

Samples:

sample_one: Sso1, [U-99% 15N], 1.2 mM

sample_conditions: ionic strength: 220; pH: 4.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_onenot availablesample_conditions
1H-15N-NOESY-HSQCsample_onenot availablesample_conditions
1H-15N-TOCSY-HSQCsample_onenot availablesample_conditions

Software:

FELIX v95 - transformation

XEasy v1.3.11 - Assignment

NMR spectrometers:

  • Varian . 600 MHz

Related Database Links:

PDB
DBJ GAA26746
EMBL CAA47959 CAA54217 CAA64255 CAA97949 CAY86728
GB AHY77951 AJP41919 AJU23363 AJU24051 AJU24725
REF NP_015092
SP P32867
TPG DAA11204
AlphaFold P32867

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks