BMRB Entry 4324

Title:
Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein.
Deposition date:
1999-03-22
Original release date:
1999-07-12
Authors:
Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.
Citation:

Citation: Karimi, Afshin; Matsumura, Masazumi; Wright, Peter; Dyson, H.. "Characterization of Monomeric and Dimeric B-domain of Staphyococcal Protein A: Sources of Stabilization of a 3-helix Bundle Protein."  J. Pept. Res. ., .-..

Assembly members:

Assembly members:
B domain of protein A, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: golden staph.   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
B domain of protein A: ADNKFNKEQQNAFYEILHLP NLNEEQRNGFIQSLKDDPSQ SANLLAEAKKLNDAQAPK

Data sets:
Data typeCount
15N chemical shifts69
1H chemical shifts411

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B domain of protein A1

Entities:

Entity 1, B domain of protein A 58 residues - Formula weight is not available

1   ALAASPASNLYSPHEASNLYSGLUGLNGLN
2   ASNALAPHETYRGLUILELEUHISLEUPRO
3   ASNLEUASNGLUGLUGLNARGASNGLYPHE
4   ILEGLNSERLEULYSASPASPPROSERGLN
5   SERALAASNLEULEUALAGLUALALYSLYS
6   LEUASNASPALAGLNALAPROLYS

Samples:

sample_1: B domain of protein A, [U-95% 15N], 1.0 – 4.0 mM

cond-1: pH: 5.0 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 1120 1136 2353 4325
PDB
EMBL CAA49867
GB AAA72944 ABX88876 ABX88877 ACX42323 EEV74738
REF WP_031789382 WP_052996913

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks