BMRB Entry 5378

Title:
NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges
Deposition date:
2002-05-17
Original release date:
2003-02-20
Authors:
Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt
Citation:

Citation: Zahn, Ralph; Guntert, Peter; von Schroetter, Christine; Wuthrich, Kurt. "NMR Structure of a Variant Human Prion Protein with Two Disulfide Bridges"  J. Mol. Biol. 326, 225-234 (2003).
PubMed: 12547204

Assembly members:

Assembly members:
human prion protein 121-230, polymer, 112 residues, 13092 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts770
13C chemical shifts321
15N chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hprp(121-230) subunit 11

Entities:

Entity 1, hprp(121-230) subunit 1 112 residues - 13092 Da.

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROILE
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETHISARGTYRPRO
5   ASNGLNVALTYRTYRARGPROCYSASPGLU
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLUARGCYSSERGLNALATYRTYRGLNARG
12   GLYSER

Samples:

sample_1: human prion protein 121-230, [U-15N; U-13C], 0.7 mM

sample_2: human prion protein 121-230 0.7 mM

Ex-cond_1: pH: 4.5 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-1H-15N/13C NOESYnot availablenot availablenot available
1H-1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

PROSA -

XEASY -

DYANA-CANDID -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 15676 17756 17780 18426 4379 4402 4434 4620 4736 5713
PDB
DBJ BAA00011 BAG32276 BAG32278 BAG32279 BAG52189
EMBL CAA58442
GB AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SP P04156 P40252
AlphaFold P40252 P04156

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks