BMRB Entry 5815

Title:
Structure of the coat protein in fd filamentous bacteriophage particles
Deposition date:
2003-06-03
Original release date:
2003-10-17
Authors:
Zeri, A.; Mesleh, M.; Nevzorov, A.; Opella, S.
Citation:

Citation: Zeri, A.; Mesleh, M.; Nevzorov, A.; Opella, S.. "Structure of the Coat Protein in fd Filamentous Bacteriophage Particles Determined by Solid-state NMR Spectroscopy"  Proc. Natl. Acad. Sci. U. S. A. 100, 6458-6463 (2003).
PubMed: 12750469

Assembly members:

Assembly members:
bacteriophage fd major coat protein, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: bacteriophage   Taxonomy ID: 12420   Superkingdom: virus   Kingdom: not available   Genus/species: Filamentous bacteriophage

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bacteriophage fd major coat protein: AEGDDPAKAAFDSLQASATE MIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:
Data typeCount
15N chemical shifts43
residual dipolar couplings43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p8 monomer1

Entities:

Entity 1, p8 monomer 50 residues - Formula weight is not available

1   ALAGLUGLYASPASPPROALALYSALAALA
2   PHEASPSERLEUGLNALASERALATHRGLU
3   METILEGLYTYRALATRPALAMETVALVAL
4   VALILEVALGLYALATHRILEGLYILELYS
5   LEUPHELYSLYSPHETHRSERLYSALASER

Samples:

sample_1: bacteriophage fd major coat protein, [U-15N], 50 mg/ml; sodium borate buffer 5 mM

sample_cond_1: pH: 8.0 na; temperature: 338 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
PISEMA-Polarizationsample_1not availablesample_cond_1
Inversion and Spin Exchange at the Magic Anglesample_1not availablesample_cond_1

Software:

tecmag vlibra - collection

FELIX v97 - processing

SCWRL v2.1 - refinement

NMR spectrometers:

  • homebuilt . 550 MHz

Related Database Links:

SWISS-PROT P69539 P69540
GenBank AAF75598 AAA32308 AAB24445 AAA32214 AAA32220
PRF 0812197K
PDB
EMBL CAA23871