BMRB Entry 5820

Title:
Tertiary structure of thiopurine methyltransferase from Pseudomonas syringae, the bacterial orthologue of a polymorphic, drug-metabolizing enzyme
Deposition date:
2003-06-06
Original release date:
2003-10-17
Authors:
Scheuermann, Thomas; Lolis, Elias; Hodsdon, Michael
Citation:

Citation: Scheuermann, Thomas; Lolis, Elias; Hodsdon, Michael. "Tertiary Structure of Thiopurine Methyltransferase from Pseudomonas syringae, a Bacterial Orthologue of a Polymorphic, Drug-metabolizing Enzyme "  J. Mol. Biol. 333, 573-585 (2003).
PubMed: 14556746

Assembly members:

Assembly members:
thiopurine methyltransferase, polymer, 203 residues, 22566.5 Da.

Natural source:

Natural source:   Common Name: Pseudomonas syringae   Taxonomy ID: 317   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas syringae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1171
13C chemical shifts792
15N chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TPMT1

Entities:

Entity 1, TPMT 203 residues - 22566.5 Da.

1   GLYSERHISGLNSERGLUVALASNLYSASP
2   LEUGLNGLNTYRTRPSERSERLEUASNVAL
3   VALPROGLYALAARGVALLEUVALPROLEU
4   CYSGLYLYSSERGLNASPMETSERTRPLEU
5   SERGLYGLNGLYTYRHISVALVALGLYALA
6   GLULEUSERGLUALAALAVALGLUARGTYR
7   PHETHRGLUARGGLYGLUGLNPROHISILE
8   THRSERGLNGLYASPPHELYSVALTYRALA
9   ALAPROGLYILEGLUILETRPCYSGLYASP
10   PHEPHEALALEUTHRALAARGASPILEGLY
11   HISCYSALAALAPHETYRASPARGALAALA
12   METILEALALEUPROALAASPMETARGGLU
13   ARGTYRVALGLNHISLEUGLUALALEUMET
14   PROGLNALACYSSERGLYLEULEUILETHR
15   LEUGLUTYRASPGLNALALEULEUGLUGLY
16   PROPROPHESERVALPROGLNTHRTRPLEU
17   HISARGVALMETSERGLYASNTRPGLUVAL
18   THRLYSVALGLYGLYGLNASPTHRLEUHIS
19   SERSERALAARGGLYLEULYSALAGLYLEU
20   GLUARGMETASPGLUHISVALTYRVALLEU
21   GLUARGVAL

Samples:

sample_1: thiopurine methyltransferase, [U-98% 13C; U-98% 15N], 1.5 mM; potassium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.05%; leupeptin 1 uM; pepstatin 1 uM; PMSF 1 uM; D20 5%

cond_set_1: pH: 6.8; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecond_set_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAC27664 EGH42918 KEZ71289 KFF83305
REF WP_024960070 WP_032661192
SP O86262
AlphaFold O86262

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks