BMRB Entry 6126

Title:
NMR Structure of the CRIB-PDZ module of Par-6
Deposition date:
2004-03-03
Original release date:
2004-05-15
Authors:
Peterson, F.; R Penkert, R.; Volkman, B.; Prehoda, K.
Citation:

Citation: Peterson, F.; Penkert, R.; Volkman, B.; Prehoda, K.. "Cdc42 Regulates the Par-6 PDZ Domain Through an Allosteric CRIB-PDZ Transition"  Mol. Cell 13, 665-676 (2004).
PubMed: 15023337

Assembly members:

Assembly members:
Par-6, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts531
15N chemical shifts127
1H chemical shifts831

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Par-61

Entities:

Entity 1, Par-6 128 residues - Formula weight is not available

1   GLYSERLYSTHRLYSALAPROSERILESER
2   ILEPROHISASPPHEARGGLNVALSERALA
3   ILEILEASPVALASPILEVALPROGLUTHR
4   HISARGARGVALARGLEULEULYSHISGLY
5   SERASPLYSPROLEUGLYPHETYRILEARG
6   ASPGLYTHRSERVALARGVALTHRALASER
7   GLYLEUGLULYSGLNPROGLYILEPHEILE
8   SERARGLEUVALPROGLYGLYLEUALAGLU
9   SERTHRGLYLEULEUALAVALASNASPGLU
10   VALILEGLUVALASNGLYILEGLUVALALA
11   GLYLYSTHRLEUASPGLNVALTHRASPMET
12   METVALALAASNSERSERASNLEUILEILE
13   THRVALLYSPROALAASNGLNARG

Samples:

sample_1: Par-6, [U-15N; U-13C], 1 mM; phosphate buffer 20 mM; sodium chloride 50 mM; sodium azide 0.05%; H2O 90%; D2O 10%

sample_2: Par-6, [U-15N], 0.5 mM; phosphate buffer 20 mM; sodium azide 0.05%; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; temperature: 303 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated aromatic NOESYnot availablenot availablesample_cond_1

Software:

XWINNMR v3.1 - collection

NMRPipe v97.027.12.56 - processing

Xeasy v1.3.13 - data analysis

Garant v2.1 - data analysis

Cyana v1.0.6 - structure solution

XPLOR-NIH v2.0.6 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 17599 17600
PDB
GB AAD15927 AAF48757 AAL28917 AAN09592 ACL90845
REF NP_573238 NP_728094 XP_001355305 XP_001963708 XP_001977590

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks