BMRB Entry 6262

Title:
NMR structure of WW domains (WW3-4) from Suppressor of Deltex
Deposition date:
2004-07-16
Original release date:
2004-09-20
Authors:
Fedoroff, O.; Avis, J.; Golovanov, A.; Baron, M.; Townson, S.
Citation:

Citation: Fedoroff, O.; Townson, S.; Golovanov, A.; Baron, M.; Avis, J.. "The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, suppressor of deltex"  J. Biol. Chem. 279, 34991-35000 (2004).
PubMed: 15173166

Assembly members:

Assembly members:
CG4244-PB, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: friut fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGEX

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts385
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CG4244-PB1

Entities:

Entity 1, CG4244-PB 88 residues - Formula weight is not available

1   GLYSERPROGLUPHEHISMETASPALALEU
2   GLYPROLEUPROASPGLYTRPGLULYSLYS
3   ILEGLNSERASPASNARGVALTYRPHEVAL
4   ASNHISLYSASNARGTHRTHRGLNTRPGLU
5   ASPPROARGTHRGLNGLYGLNGLUVALSER
6   LEUILEASNGLUGLYPROLEUPROPROGLY
7   TRPGLUILEARGTYRTHRALAALAGLYGLU
8   ARGPHEPHEVALASPHISASNTHRARGARG
9   THRTHRPHEGLUASPPROARGPRO

Samples:

sample_1: CG4244-PB, [U-15N], 0.5 mM; Arg/Glu 50 mM; H2O 90%; D2O 10%; Na/KCl 150 mM

sample_cond_1: pH: 7.1; temperature: 288 K; ionic strength: 150 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
HSQC in liquid crystal medianot availablenot availablenot available

Software:

ARIA v1.2 - refinement

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
GB AAL39551

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks