BMRB Entry 6355

Title:
Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8.
Deposition date:
2004-10-14
Original release date:
2006-04-06
Authors:
Baran, M.; Huang, Y.; Acton, T.; Xiao, R.; Montelione, G.
Citation:

Citation: Baran, M.; Huang, Y.; Acton, T.; Xiao, R.; Montelione, G.. "Solution Structure Of The Staphylococcus Epidermis Protein SE0936. Northest Strucutral Genomics Consortium Target SeR8. "  .

Assembly members:

Assembly members:
SE0936, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus epidermidis   Taxonomy ID: 1282   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus epidermidis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts573
13C chemical shifts348
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SE09361

Entities:

Entity 1, SE0936 88 residues - Formula weight is not available

1   METPROTHRGLUASNPROTHRMETPHEASP
2   GLNVALALAGLUVALILEGLUARGLEUARG
3   PROPHELEULEUARGASPGLYGLYASPCYS
4   THRLEUVALASPVALGLUASPGLYILEVAL
5   LYSLEUGLNLEUHISGLYALACYSGLYTHR
6   CYSPROSERSERTHRILETHRLEULYSALA
7   GLYILEGLUARGALALEUHISGLUGLUVAL
8   PROGLYVALILEGLUVALGLUGLNVALPHE
9   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: SE0936, [U-5% 13C; U-100% 15N], 8 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H20 95%

sample_2: SE0936, [U-100% 13C; U-100% 15N], 8 mM; NaN3 0.02%; DTT 10 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; D2O 5%; H20 95%

sample_cond_1: pH: 6.5; temperature: 293 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
C13-HSQCnot availablenot availablenot available
NH-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCABnot availablenot availablenot available
N15-NOESYnot availablenot availablenot available
C13-NOESYnot availablenot availablenot available

Software:

X-PLOR v2.9.7 - structure solution, refinement

NMRPipe v2.1 - processing

AutoAssign v1.14 - data analysis

AutoStructure v2.0 - data analysis

SPINS v5.0 - data analysis

HYPER v3.2 - data analysis

sparky v3.106 - data analysis

VNMR v6.1 - collection

xwin-nmr - collection

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE05324
EMBL CCB54437 CDI72254 CDM13169
GB AAO04227 AAW37908 AAW53911 ADC88025 AGC91125
REF NP_764185 WP_001831958 WP_002433501 WP_002456117 WP_010956550

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks