BMRB Entry 6386

Title:
Structural and Functional Study of Anemonia Elastase Inhibitor, a "Non-classical" Kazal-type Inhibitor from Anemonia sulcata
Deposition date:
2004-11-15
Original release date:
2005-09-08
Authors:
Hemmi, Hikaru; Kumazaki, Takashi; Yoshizawa-Kumagaye, Kumiko; Nishiuchi, Yuji; Yoshida, Takuya; Ohkubo, Tadayasu; Kobayashi, Yuji
Citation:

Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yoshizawa-Kumagaye, Kumiko; Nishiuchi, Yuji; Yoshida, Takuya; Ohkubo, Tadayasu; Kobayashi, Yuji. "Structural and Functional Study of an Anemonia Elastase Inhibitor, a "Nonclassical" Kazal-Type Inhibitor from Anemonia sulcata."  Biochemistry 44, 9626-9636 (2005).
PubMed: 16008348

Assembly members:

Assembly members:
anemonia elastase inhibitor, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anemonia sulcata   Taxonomy ID: 6108   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Anemonia sulcata

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
anemonia elastase inhibitor: KPDCPLICTMQYDPVCGSDG ITYGNACMLLGASCRSDTPI ELVHKGRC

Data sets:
Data typeCount
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aei monomer1

Entities:

Entity 1, aei monomer 48 residues - Formula weight is not available

1   LYSPROASPCYSPROLEUILECYSTHRMET
2   GLNTYRASPPROVALCYSGLYSERASPGLY
3   ILETHRTYRGLYASNALACYSMETLEULEU
4   GLYALASERCYSARGSERASPTHRPROILE
5   GLULEUVALHISLYSGLYARGCYS

Samples:

sample_1: anemonia elastase inhibitor3.0 – 3.5 mM

Ex-cond_1: pH: 2.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H DQFCOSYnot availablenot availablenot available
1H-1H ECOSYnot availablenot availablenot available
1H-1H ROESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

xwinnmr - processing

SPARKY - assignment, peak picking

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
SWISS-PROT P16895