BMRB Entry 6489

Title:
Chemical shift assignments for subunit c of the F1Fo ATP Synthase from Bacillus PS3
Deposition date:
2005-02-04
Original release date:
2006-04-24
Authors:
Nakano, Takayuki; Ikegami, Takahisa; Suzuki, Toshiharu; Yoshida, Masasuke; Akutsu, Hideo
Citation:

Citation: Nakano, Takayuki; Ikegami, Takahisa; Suzuki, Toshiharu; Yoshida, Masasuke; Akutsu, Hideo. "A New Solution Structure of ATP Synthase Subunit c from Thermophilic Bacillus PS3, Suggesting a Local Conformational Change for H(+)-Translocation"  J. Mol. Biol. ., .-. (2006).
PubMed: 16497328

Assembly members:

Assembly members:
F1Fo-ATP synthase subunit c, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermophilic Bacillus PS3   Taxonomy ID: 2334   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus Bacillus sp. PS3

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
F1Fo-ATP synthase subunit c: MSLGVLAAAIAVGLGALGAG IGNGLIVSRTIEGIARQPEL RPVLQTTMFIGVALVEALPI IGVVFSFIYLGR

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts508
13C chemical shifts306
15N chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1F1Fo-c1

Entities:

Entity 1, F1Fo-c 72 residues - Formula weight is not available

1   METSERLEUGLYVALLEUALAALAALAILE
2   ALAVALGLYLEUGLYALALEUGLYALAGLY
3   ILEGLYASNGLYLEUILEVALSERARGTHR
4   ILEGLUGLYILEALAARGGLNPROGLULEU
5   ARGPROVALLEUGLNTHRTHRMETPHEILE
6   GLYVALALALEUVALGLUALALEUPROILE
7   ILEGLYVALVALPHESERPHEILETYRLEU
8   GLYARG

Samples:

sample_1: F1Fo-ATP synthase subunit c 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%

sample_2: F1Fo-ATP synthase subunit c, [U-15N], 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%

sample_3: F1Fo-ATP synthase subunit c, [U-13C; U-15N], 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%

condition_1: pH: 2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-1N-HSQCsample_2not availablecondition_1
2D_NOESYsample_1not availablecondition_1
CBCA(CO)NHsample_3not availablecondition_1
CBCANHsample_3not availablecondition_1
HNCOsample_3not availablecondition_1
HN(CA)COsample_3not availablecondition_1
HBHANHsample_3not availablecondition_1
HBHA(CO)NHsample_3not availablecondition_1
H(CCO)NHsample_3not availablecondition_1
C(CO)NHsample_3not availablecondition_1

Software:

NMRPipe v2.2 - processing

Sparky v3.106 - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 11550
PDB
DBJ BAA07244 BAA07251 BAD77648 GAD12918 GAJ57176
EMBL CAA30649
GB ACX79968 ADI28261 ADU95796 AEV21076 AGE23948
PIR LWHWA3
REF WP_011232830 WP_014196950 YP_003254450 YP_003672838 YP_004133939
SP P00845 P41015 P42011 Q5KUI8
AlphaFold P00845 P41015 P42011 Q5KUI8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks