BMRB Entry 6520

Title:
1H, 15N Chemical shift assignments for recombinant elicitor protein PcF from the oomycete pathogen P. cactorum
Deposition date:
2005-02-21
Original release date:
2009-05-27
Authors:
Nicastro, Giuseppe; Orsomando, Giuseppe; Desario, F.; Ferrari, Elena; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio
Citation:

Citation: Nicastro, G.; Orsomando, Giuseppe; Desario, F.; Ferrari, E.; Manconi, L.; Spisni, Alberto; Ruggeri, Silverio. "The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum displays a helix-loop-helix fold and reveals calcium binding ability"  .

Assembly members:

Assembly members:
elicitor protein Pcf from the oomycete pathogen P. cactorum, polymer, 52 residues, 6214 Da.

Natural source:

Natural source:   Common Name: Pichia Pastoris   Taxonomy ID: 4922   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phytophthora cactorum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
elicitor protein Pcf from the oomycete pathogen P. cactorum: EDPLYCQAIGCPTLYSEANL AVSKECRDQGKLGDDFHRCC EEQCGSTTPASA

Data sets:
Data typeCount
15N chemical shifts49
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pcf monomer1

Entities:

Entity 1, Pcf monomer 52 residues - 6214 Da.

1   GLUASPPROLEUTYRCYSGLNALAILEGLY
2   CYSPROTHRLEUTYRSERGLUALAASNLEU
3   ALAVALSERLYSGLUCYSARGASPGLNGLY
4   LYSLEUGLYASPASPPHEHISARGCYSCYS
5   GLUGLUGLNCYSGLYSERTHRTHRPROALA
6   SERALA

Samples:

sample_1: Pcf monomer, [U-15N], 0.65 mM

conditions_1: pH: 5.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D-TOCSY-HSQCnot availablenot availablenot available
3D-NOESY-HSQCnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available
2D-TOSCYnot availablenot availablenot available
2D-HSQC-15Nnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAK63068

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks