BMRB Entry 6560

Title:
The NMR solution structure of a redesigned apocytochrome b562:Rd-apocyt b562
Deposition date:
2005-03-18
Original release date:
2005-08-30
Authors:
Feng, H.; Takei, J.; Lipsitz, R.; Tjandra, N.; Bai, Y.
Citation:

Citation: Feng, H.; Takei, J.; Lipsitz, R.; Tjandra, N.; Bai, Y.. "Specific non-native hydrophobic interactions in a hidden folding intermediate: implications for protein folding"  Biochemistry 42, 12461-12465 (2003).
PubMed: 14580191

Assembly members:

Assembly members:
redesigned apocytochrome B562, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homon sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts331
15N chemical shifts110
1H chemical shifts713
coupling constants99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1redesigned apocytochrome B5621

Entities:

Entity 1, redesigned apocytochrome B562 106 residues - Formula weight is not available

1   ALAASPLEUGLUASPASNTRPGLUTHRLEU
2   ASNASPASNLEULYSVALILEGLULYSALA
3   ASPASNALAALAGLNVALLYSASPALALEU
4   THRLYSMETARGALAALAALALEUASPALA
5   GLNLYSALATHRPROPROLYSLEUGLUASP
6   LYSSERPROASPSERPROGLUMETLYSASP
7   PHEARGHISGLYPHEASPILELEUVALGLY
8   GLNILEASPASPALALEULYSLEUALAASN
9   GLUGLYLYSVALLYSGLUALAGLNALAALA
10   ALAGLUGLNLEULYSTHRTHRILEARGALA
11   TYRASNGLNLYSTYRGLY

Samples:

sample_1: redesigned apocytochrome B562, [U-15N; U-13C], 1.5 mM; Sodium acetate 50 mM; H2O 92.5%; D2O 7.5%

sample_2: redesigned apocytochrome B562, [U-13C], 1.5 mM; Sodium acetate 50 mM; H2O 92.5%; D2O 7.5%

sample_cond_1: pH: 4.84; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D HSQCnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CO)NHnot availablenot availablenot available

Software:

X-PLOR vXPLOR-NIH-2.9 - refinement, structure solution

NMRPipe - processing

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

PDB
EMBL CDX09681
GB AAN45672 AIL38642 AIL43578 EFS10896 EGJ79391
REF NP_709965 WP_005086530

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks