BMRB Entry 6580

Title:
assignment of mouse socs3 by NMR
Deposition date:
2005-04-04
Original release date:
2005-12-16
Authors:
Babon, Jeffrey; Yao, Shenggen; deSouza, David; Harrison, Christopher; Fabri, Louis; Liepinsch, Edvards; Otting, Gottfried; Baca, Manuel; Norton, Raymond
Citation:

Citation: Babon, Jeffrey; Yao, Shenggen; deSouza, David; Harrison, Christopher; Fabri, Louis; Liepinsh, Edvards; Scrofani, Sergio; Baca, Manuel; Norton, Raymond. "Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain."  FEBS J. 272, 6120-6130 (2005).
PubMed: 16302975

Assembly members:

Assembly members:
mouse socs3, polymer, 164 residues, Formula weight is not available
gp130 phosphopeptide, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: domestic mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts146
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse socs31
2gp130 phosphopeptide2

Entities:

Entity 1, mouse socs3 164 residues - Formula weight is not available

1   LEULYSTHRPHESERSERLYSSERGLUTYR
2   GLNLEUVALVALASNALAVALARGLYSLEU
3   GLNGLUSERGLYPHETYRTRPSERALAVAL
4   THRGLYGLYGLUALAASNLEULEULEUSER
5   ALAGLUPROALAGLYTHRPHELEUILEARG
6   ASPSERSERASPGLNARGHISPHEPHETHR
7   LEUSERVALLYSTHRGLNSERGLYTHRLYS
8   ASNLEUARGILEGLNCYSGLUGLYGLYSER
9   PHESERLEUGLNSERASPPROARGSERTHR
10   GLNPROVALPROARGPHEASPCYSVALLEU
11   LYSLEUVALHISHISTYRMETPROPROPRO
12   GLYTHRPROSERPHESERLEUPROPROTHR
13   GLUPROSERSERGLUVALPROGLUGLNPRO
14   PROALAGLNALALEUPROGLYSERTHRPRO
15   LYSARGALATYRTYRILETYRSERGLYGLY
16   GLULYSILEPROLEUVALLEUSERARGPRO
17   LEUSERSERASN

Entity 2, gp130 phosphopeptide 15 residues - Formula weight is not available

1   SERTHRALASERTHRVALGLUPTRSERTHR
2   VALVALHISSERGLY

Samples:

sample_1: mouse socs3, [U-13C; U-15N], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-13C; U-15N], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_2: mouse socs3, [U-13C], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-13C], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_3: mouse socs3, [U-15N], 0.4 – 0.5 mM; gp130 phosphopeptide, [U-15N], 0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

sample_4: mouse socs30.4 – 0.5 mM; gp130 phosphopeptide0.4 – 0.5 mM; water mM; glutamic acid 50 mM; arginine 50 mM

conditions_1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availableconditions_1
13C-HSQCnot availablenot availableconditions_1
13C-NOESY-HSQCnot availablenot availableconditions_1
13C-HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
15N-HSQC-NOESYnot availablenot availableconditions_1
2D 1H NOESYnot availablenot availableconditions_1
1H TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz
  • . . 600 MHz
  • . . 800 MHz

Related Database Links:

PDB
DBJ BAC32977 BAE23929 BAE29797 BAE30668 BAE31222
EMBL CAB56083 CAM19105
GB AAB62403 AAC26223 AAH52031 AAK60601 EDL34646
REF NP_031733 NP_446017
SP O35718 O88583
AlphaFold O35718 O88583

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks