BMRB Entry 6600

Title:
Solution Structure of Bacillus subtilis s-BCCP apo-form
Deposition date:
2005-04-14
Original release date:
2007-01-26
Authors:
Cui, Gaofeng; Xia, Bin
Citation:

Citation: Cui, Gaofeng; Nan, Beiyan; Hu, Jicheng; Wang, Yiping; Jin, Changwen; Xia, Bin. "Identification and solution structures of a single-domain biotin/lipoyl attachment protein from bacillus subtilis"  J. Biol. Chem. 281, 20598-20607 (2006).
PubMed: 16699181

Assembly members:

Assembly members:
Biotin Carboxyl Carrier Protein, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Biotin Carboxyl Carrier Protein: TVSIQMAGNLWKVHVKAGDQ IEKGQEVAILESMKMEIPIV ADRSGIVKEVKKKEGDFVNE GDVLLELSNSTQ

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts78
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Biotin Carboxyl Carrier Protein1

Entities:

Entity 1, Biotin Carboxyl Carrier Protein 72 residues - Formula weight is not available

1   THRVALSERILEGLNMETALAGLYASNLEU
2   TRPLYSVALHISVALLYSALAGLYASPGLN
3   ILEGLULYSGLYGLNGLUVALALAILELEU
4   GLUSERMETLYSMETGLUILEPROILEVAL
5   ALAASPARGSERGLYILEVALLYSGLUVAL
6   LYSLYSLYSGLUGLYASPPHEVALASNGLU
7   GLYASPVALLEULEUGLULEUSERASNSER
8   THRGLN

Samples:

sample_1: Biotin Carboxyl Carrier Protein, [U-13C; U-15N], 1.2 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

NMRPipe v2.1 - processing

NMRView v5 - data analysis

CYANA v1.0.6 - structure solution

AMBER v7.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 6599
PDB
DBJ BAI85513 BAM52478 BAM58054 GAK81515
EMBL CAX52627 CEI57017 CEJ77441
GB ADV92730 AEP91001 AFQ57759 AGE63668 AGG61199
REF WP_003245519 WP_014476962 WP_014479957 WP_015383786 WP_031600576
SP C0H419
AlphaFold C0H419

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks