BMRB Entry 6690

Title:
Partial 1H, 13C, and 15N Chemical Shift Assignments for PSI AB box region in complex with U1-70k protein proline-rich peptide
Deposition date:
2005-06-15
Original release date:
2007-01-26
Authors:
Ignjatovic, Tijana; Yang, Ji-Chun; Butler, Jonathan; Neuhaus, David; Nagai, Kiyoshi
Citation:

Citation: Ignjatovic, Tijana; Yang, Ji-Chun; Butler, Jonathan; Neuhaus, David; Nagai, Kiyoshi. "Structural Basis of the Interaction Between P-element Somatic Inhibitor and U1-70k Essential for the Alternative Splicing of P-element Transposase"  J. Mol. Biol. 351, 52-65 (2005).
PubMed: 15990112

Assembly members:

Assembly members:
PSI AB box, polymer, 93 residues, Formula weight is not available
U1-70k peptide, polymer, 21 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Protista   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts128
15N chemical shifts59
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PSI AB box1
2U1-70k proline-rich peptide2

Entities:

Entity 1, PSI AB box 93 residues - Formula weight is not available

1   SERASPTYRSERALAGLNTRPILEGLUTYR
2   TYRLYSGLNMETGLYSERHISARGGLUALA
3   GLUMETILEGLUGLNGLNMETLYSALALYS
4   GLNALAGLYGLYSERSERGLYPROVALGLN
5   GLNGLNGLNPROGLNGLNGLNGLNGLNSER
6   GLNGLNGLNTHRGLYGLYALAGLYALAGLY
7   GLYALAASPTYRSERALAGLNTRPALAGLU
8   TYRTYRARGSERVALGLYLYSILEGLUGLU
9   ALAGLUALAILEGLULYSTHRLEULYSASN
10   LYSGLNASN

Entity 2, U1-70k proline-rich peptide 21 residues - Formula weight is not available

1   ARGPROPROPROALAHISHISASNMETPHE
2   SERVALPROPROPROPROILELEUGLYARG
3   GLY

Samples:

sample_1: PSI AB box, [U-15N], 0.4 – 0.42 mM; U1-70k peptide0.4 – 0.42 mM; sodium phosphate 10 mM; sodium chloride 150 mM; DTT 5 mM

sample_2: PSI AB box, [U-13C; U-15N], 0.4 – 0.45 mM; U1-70k peptide0.4 – 0.45 mM; sodium phosphate 10 mM; sodium chloride 150 mM; DTT 5 mM

conditions_1: pH: 6.5; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availableconditions_1
2D TOCSYsample_1not availableconditions_1
2D 15N HSQCsample_1not availableconditions_1
2D 13C HSQCsample_2not availableconditions_1
3D HNCAsample_2not availableconditions_1
3D HN(CO)CAsample_2not availableconditions_1
3D CBCA(CO)NHsample_2not availableconditions_1
3D CBCANHsample_2not availableconditions_1
3D HBHA(CO)NHsample_2not availableconditions_1
3D HBHANHsample_2not availableconditions_1
3D HCCH COSYsample_2not availableconditions_1
3D HCCH TOCSYsample_2not availableconditions_1
3D 15N NOESY-HSQCsample_1not availableconditions_1
3D 13C NOESY-HSQCsample_2not availableconditions_1
2D (13C, 15N) Filtered NOESYsample_2not availableconditions_1
3D (13C, 15N) Filtered 13C HSQC NOESYsample_2not availableconditions_1

Software:

xwinnmr v3.1, Bruker - analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAA28859 AAF52471 AAL29049 AAL90360 ACL85981
REF NP_001260173 NP_477205 XP_001962392 XP_001970105 XP_002036020
SP P17133
AlphaFold P17133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks