BMRB Entry 6753

Title:
Backbone 1H,13C, and 15N chemical shift assignments for the PH1 domain of alpha-syntrophin
Deposition date:
2005-07-27
Original release date:
2007-01-29
Authors:
Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie
Citation:

Citation: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie. "Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin."  EMBO J. 24, 3985-3995 (2005).
PubMed: 16252003

Assembly members:

Assembly members:
split PH domain, polymer, 178 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts162
1H chemical shifts1066

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1split PH domain1

Entities:

Entity 1, split PH domain 178 residues - Formula weight is not available

1   ALASERGLYARGARGALAPROARGTHRGLY
2   LEULEUGLULEUARGCYSGLYALAGLYSER
3   GLYALAGLYGLYGLUARGTRPGLNARGVAL
4   LEULEUSERLEUALAGLUASPALALEUTHR
5   VALSERPROALAASPGLYGLUPROGLYPRO
6   GLUPROGLUPROALAGLNLEUASNGLYALA
7   ALAGLUPROGLYALAALAPROPROGLNLEU
8   PROGLUALALEULEULEUGLNARGGLUVAL
9   SERPROTYRPHELYSASNSERALAGLYGLY
10   THRSERVALGLYTRPASPSERPROPROALA
11   SERPROLEUGLNARGGLNPROSERSERPRO
12   GLYPROGLNPROARGASNLEUSERGLUALA
13   LYSHISVALSERLEULYSMETALATYRVAL
14   SERARGARGCYSTHRPROTHRASPPROGLU
15   PROARGTYRLEUGLUILECYSALAALAASP
16   GLYGLNASPALAVALPHELEUARGALALYS
17   ASPGLUALASERALAARGSERTRPALAGLY
18   ALAILEGLNALAGLNILEGLYTHR

Samples:

sample_1: split PH domain, [U-13C; U-15N], 1 mM

sample_2: split PH domain, [U-15N], 1 mM

conditions_1: pH: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15C-separated NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks