BMRB Entry 6803

Title:
1H, 13C, and 15N Chemical Shift Assignments for the periplasmic signaling domain of FecA from Escherichia coli
Deposition date:
2005-08-31
Original release date:
2005-12-22
Authors:
Garcia-Herrero, Alicia; Vogel, Hans
Citation:

Citation: Garcia-Herrero, Alicia; Vogel, Hans. "Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli"  Mol. Microbiol. 58, 1226-1237 (2005).
PubMed: 16313612

Assembly members:

Assembly members:
NTFecA, polymer, 104 residues, 11138 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts381
15N chemical shifts107
1H chemical shifts672

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTFecA1

Entities:

Entity 1, NTFecA 104 residues - 11138 Da.

The polypeptide construct contains two extra residues (LE) and a His6tag at the N-terminal end

1   ALAGLNVALASNILEALAPROGLYSERLEU
2   ASPLYSALALEUASNGLNTYRALAALAHIS
3   SERGLYPHETHRLEUSERVALASPALASER
4   LEUTHRARGGLYLYSGLNSERASNGLYLEU
5   HISGLYASPTYRASPVALGLUSERGLYLEU
6   GLNGLNLEULEUASPGLYSERGLYLEUGLN
7   VALLYSPROLEUGLYASNASNSERTRPTHR
8   LEUGLUPROALAPROALAPROLYSGLUASP
9   ALALEUTHRVALVALGLYASPTRPLEUGLY
10   ASPALAARGGLUASNASPLEUGLUHISHIS
11   HISHISHISHIS

Samples:

sample_1: NTFecA, [U-15N], 1 mM; Sodium phosphate 50 mM

sample_2: NTFecA, [U-15N; U-13C], 1 mM; Sodium Phosphate 50 mM

conditions_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D Protonnot availablenot availableconditions_1
2D Proton-Proton NOESY (D2O)not availablenot availableconditions_1
2D 1H-15N HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
CBCANHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
CCCONH-TOCSYnot availablenot availableconditions_1
HBHA(CO)NHnot availablenot availableconditions_1
HCCCONH-TOCSYnot availablenot availableconditions_1
3D 13C separated NOESYnot availablenot availableconditions_1
3D 15N separated NOESYnot availablenot availableconditions_1
Steady State 15N Heteronuclear noenot availablenot availableconditions_1
T2 15Nnot availablenot availableconditions_1
T2 15Nnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB
    DBJ BAE78282 BAI28590 BAJ46002
    EMBL CAQ34637 CAR06012 CAR11109 CAR15972 CAR20866
    GB AAA23760 AAA23768 AAA97187 AAC77247 AAL08456
    REF NP_418711 WP_000188246 WP_000188247 WP_000188248 WP_000188249
    SP P13036
    AlphaFold P13036 P13036

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks