BMRB Entry 6844

Title:
Solution Structure of Drosophila melanogaster SNF RBD2
Deposition date:
2005-09-29
Original release date:
2009-10-20
Authors:
Cui, G.; Li, C.; Jin, C.; Xia, B.
Citation:

Citation: Jicheng, Hu; Gaofeng, Cui; Congmin, Li; Cong, Liu; Erchang, Shang; Luhua, Lai; Changwen, Jin; Jiwu, Wang; Bin, Xia. "Structure and Novel Functional Mechanism of Drosophila SNF in Sex-Lethal Splicing"  PLoS One 4, 6890-. (2009).
PubMed: 19727396

Assembly members:

Assembly members:
U1 small nuclear ribonucleoprotein A, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U1 small nuclear ribonucleoprotein A: AQTEQPPNQILFLTNLPEET NEMMLSMLFNQFPGFKEVRL VPNRHDIAFVEFTTELQSNA AKEALQGFKITPTHAMKITF AKK

Data sets:
Data typeCount
13C chemical shifts339
15N chemical shifts90
1H chemical shifts601

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U1 small nuclear ribonucleoprotein A1

Entities:

Entity 1, U1 small nuclear ribonucleoprotein A 83 residues - Formula weight is not available

1   ALAGLNTHRGLUGLNPROPROASNGLNILE
2   LEUPHELEUTHRASNLEUPROGLUGLUTHR
3   ASNGLUMETMETLEUSERMETLEUPHEASN
4   GLNPHEPROGLYPHELYSGLUVALARGLEU
5   VALPROASNARGHISASPILEALAPHEVAL
6   GLUPHETHRTHRGLULEUGLNSERASNALA
7   ALALYSGLUALALEUGLNGLYPHELYSILE
8   THRPROTHRHISALAMETLYSILETHRPHE
9   ALALYSLYS

Samples:

sample_1: U1 small nuclear ribonucleoprotein A, [U-15N; U-13C], 1 mM; phosphate buffer 50 mM; EDTA 1 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 250 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

NMRPipe v2.1 - processing

NMRView v5 - data analysis

CYANA v1.0.6 - structure solution

AMBER v7.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
GB AAA28441 AAA28903 AAF46017 AAL29039 ACL84772
REF NP_511045 XP_001355411 XP_001964014 XP_001976948 XP_001992159
SP P43332
AlphaFold P43332

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks