BMRB Entry 6914

Title:
Solution structure of the Wilson ATPase N-domain in the presence of ATP
Deposition date:
2005-12-01
Original release date:
2007-02-09
Authors:
Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Morgan, C.; Markley, J.; Lutsenko, S.
Citation:

Citation: Dmitriev, O.; Tsivkovski, R.; Abildgaard, F.; Lutsenko, S.. "NMR assignment of the Wilson disease associated protein N-domain"  J. Biomol. NMR 36, 61-61 (2006).
PubMed: 16868859

Assembly members:

Assembly members:
Wilson Disease ATPase N-domain, polymer, 165 residues, 17292 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts645
15N chemical shifts164
1H chemical shifts1019

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WILSON DISEASE ATPASE, N-domain1

Entities:

Entity 1, WILSON DISEASE ATPASE, N-domain 165 residues - 17292 Da.

1   ALAGLYHISMETVALPROARGVALMETARG
2   VALLEULEULEUGLYASPVALALATHRLEU
3   PROLEUARGLYSVALLEUALAVALVALGLY
4   THRALAGLUALASERSERGLUHISPROLEU
5   GLYVALALAVALTHRLYSTYRCYSLYSGLU
6   GLULEUGLYTHRGLUTHRLEUGLYTYRCYS
7   THRASPPHEGLNALAVALPROGLYCYSGLY
8   ILEGLYCYSLYSVALSERASNVALGLUGLY
9   ILELEUALAHISSERGLUARGPROLEUSER
10   ALAPROALASERHISLEUASNGLUALAGLY
11   SERLEUPROALAGLULYSASPALAVALPRO
12   GLNTHRPHESERVALLEUILEGLYASNARG
13   GLUTRPLEUARGARGASNGLYLEUTHRILE
14   SERSERASPVALSERASPALAMETTHRASP
15   HISGLUMETLYSGLYGLNTHRALAILELEU
16   VALALAILEASPGLYVALLEUCYSGLYMET
17   ILEALAILEALAASP

Samples:

sample_1: Wilson Disease ATPase N-domain, [U-99% 13C; U-95% 15N], 0.75 mM; sodium phosphate 50 mM; DTT 5 mM; NaN3 50 mM; ATP 5 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
4D 13C/15N-separated NOESYsample_1not availablesample_cond_1

Software:

xwinnmr v3.5 - collection

FELIX v2000 - data analysis

CYANA v1.0 - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 16761
PDB
DBJ BAD92698 BAG63463
GB AAA79212 AAA92667 AAB52902 AAI17201 AAI43974
PIR S40525
PRF 2001422A
REF NP_000044 NP_001005918 NP_001230111 XP_001103242 XP_003270167
SP P35670
AlphaFold P35670

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks