BMRB Entry 6916

Title:
NMR structure of the C-terminal domain (dimer) of HPV45 oncoprotein E7
Deposition date:
2005-12-05
Original release date:
2007-11-15
Authors:
Ohlenschlager, O.; Gorlach, M.
Citation:

Citation: Ohlenschlager, O.; Seiboth, T.; Zengerling, H.; Briese, L.; Marchanka, A.; Ramachandran, R.; Baum, M.; Korbas, M.; Meyer-Klaucke, W.; Durst, M.; Gorlach, M.. "Solution structure of the partially folded high-risk human papilloma virus 45 oncoprotein E7"  Oncogene 25, 5953-5959 (2006).
PubMed: 16636661

Assembly members:

Assembly members:
Protein E7, polymer, 56 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human papillomavirus   Taxonomy ID: 10566   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available Human papillomavirus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts241
15N chemical shifts56
1H chemical shifts380

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein E7, chain 11
2Protein E7, chain 21
3ZINC ION, 12
4ZINC ION, 22

Entities:

Entity 1, Protein E7, chain 1 56 residues - Formula weight is not available

1   GLYSERHISMETALAGLUPROGLNARGHIS
2   LYSILELEUCYSVALCYSCYSLYSCYSASP
3   GLYARGILEGLULEUTHRVALGLUSERSER
4   ALAGLUASPLEUARGTHRLEUGLNGLNLEU
5   PHELEUSERTHRLEUSERPHEVALCYSPRO
6   TRPCYSALATHRASNGLN

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Protein E7, [U-15N], 1 mM; d-TRIS 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: Protein E7, [U-13C; U-15N], 1 mM; d-TRIS 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 120 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1

Software:

VNMR v6.1 rev. C - processing

XEASY v1.3.9 - data analysis

CYANA v2.1 - structure solution

OPAL v2.6 - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

SWISS-PROT P21736
GenBank ABP99833 ABP99825 ABP99817 ABP99809 AAA46974
EMBL CAB44707 CAA73661 CAA52574
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks