BMRB Entry 6919

Title:
Letter to the Editor: 1H, 13C and 15N resonance assignments of the region 655-775 of the human MAN1
Deposition date:
2005-12-07
Original release date:
2005-12-16
Authors:
Caputo, Sandrine; Feng, Lin; Gilquin, Bernard; Worman, Howard; Zinn-Justin, Sophie; Couprie, Joel
Citation:

Citation: Caputo, Sandrine; Lin, Feng; Gilquin, Bernard; Worman, Howard; Zinn-Justin, Sophie; Couprie, Joel. "NMR Assignment of Region 655-775 of Human MAN1"  J. Biomol. NMR 36, 2-2 (2006).
PubMed: 16601860

Assembly members:

Assembly members:
MAN1 655-775, polymer, 133 residues, 15892.19 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts99
1H chemical shifts707

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAN1 655-775 domain1

Entities:

Entity 1, MAN1 655-775 domain 133 residues - 15892.19 Da.

1   GLYSERPROGLUPHEARGTRPTHRLYSGLU
2   GLUGLUGLUTHRARGGLNMETTYRASPMET
3   VALVALLYSILEILEASPVALLEUARGSER
4   HISASNGLUALACYSGLNGLUASNLYSASP
5   LEUGLNPROTYRMETPROILEPROHISVAL
6   ARGASPSERLEUILEGLNPROHISASPARG
7   LYSLYSMETLYSLYSVALTRPASPARGALA
8   VALASPPHELEUALAALAASNGLUSERARG
9   VALARGTHRGLUTHRARGARGILEGLYGLY
10   ALAASPPHELEUVALTRPARGTRPILEGLN
11   PROSERALASERCYSASPLYSILELEUVAL
12   ILEPROSERLYSVALTRPGLNGLYGLNALA
13   PHEHISLEUASPARGARGLEUGLUARGPRO
14   HISARGASP

Samples:

MAN1_N15: MAN1655-775, [U-99% 15N; U-10% 2H], 0.8 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 2 mM; TSP 2 mM; NaN3 0.01%; D2O 10%

MAN1_N15_C13: MAN1655-775, [U-99% 15N; U-99% 13C; U10% 2H], 0.8 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 2 mM; TSP 2 mM; NaN3 0.01%; D2O 10%

MAN1_N15_C13_D20: MAN1655-775, [U-99% 15N; U-99% 13C; U-100% 2H], 0.8 mM; Phosphate 50 mM; NaCl 150 mM; EDTA 1 mM; TCEP 2 mM; TSP 2 mM; NaN3 0.01%; D2O 10%

conditions_1: pH: 6; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
(HCA)CO(CA)NHnot availablenot availableconditions_1
CBCACOHAnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1
HBHA(CO)NHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HCCH-COSYnot availablenot availableconditions_1
13C_HSQC_NOESYnot availablenot availableconditions_1
15N_HSQC-NOESYnot availablenot availableconditions_1

Software:

FELIX -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks