BMRB Entry 7008

Title:
Second Ca2+ binding domain of the Na,Ca-exchanger (NCX1)
Deposition date:
2006-02-28
Original release date:
2006-04-11
Authors:
Hilge, M.; Vuister, G.; Aelen, J.
Citation:

Citation: Hilge, M.; Aelen, J.; Vuister, G.. "Ca(2+) regulation in the na(+)/ca(2+) exchanger involves two markedly different ca(2+) sensors"  Mol. Cell. 22, 15-25 (2006).
PubMed: 16600866

Assembly members:

Assembly members:
Sodium/calcium exchanger 1, polymer, 165 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts148
1H chemical shifts1044

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sodium/calcium exchanger 11
2CALCIUM (II) ION2

Entities:

Entity 1, Sodium/calcium exchanger 1 165 residues - Formula weight is not available

1   METSERHISHISHISHISHISHISHISALA
2   GLYILEPHETHRPHEGLUGLUPROVALTHR
3   HISVALSERGLUSERILEGLYILEMETGLU
4   VALLYSVALLEUARGTHRSERGLYALAARG
5   GLYASNVALILEVALPROTYRLYSTHRILE
6   GLUGLYTHRALAARGGLYGLYGLYGLUASP
7   PHEGLUASPTHRCYSGLYGLULEUGLUPHE
8   GLNASNASPGLUILEVALLYSTHRILESER
9   VALLYSVALILEASPASPGLUGLUTYRGLU
10   LYSASNLYSTHRPHEPHELEUGLUILEGLY
11   GLUPROARGLEUVALGLUMETSERGLULYS
12   LYSGLYGLYPHETHRILETHRGLUGLUTYR
13   ASPASPLYSGLNPROLEUTHRSERLYSGLU
14   GLUGLUGLUARGARGILEALAGLUMETGLY
15   ARGPROILELEUGLYGLUHISTHRLYSLEU
16   GLUVALILEILEGLUGLUSERTYRGLUPHE
17   LYSSERTHRVALASP

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Sodium/calcium exchanger 1, [U-13C; U-15N], 0.5 – 1.0 mM; HEPES 20 mM; beta-Mercaptoethanol buffer 20 mM; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 306 K

Experiments:

NameSampleSample stateSample conditions
3D 13C separated aliphatic NOESYsample_1not availablesample_cond_1
3D 13C separated aromatic NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

NMRPipe v2.1 - processing

XEASY v1.3.13 - data analysis

CYANA v2.0 - structure solution

X-PLOR vNIH version 2.9.7 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA19128 ELK14758

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks