BMRB Entry 7092

Title:
Chemical shifts of Vigna radiata Defensin 2 from Mung bean
Deposition date:
2006-05-02
Original release date:
2007-05-04
Authors:
Lin, Ku-Feng; Lyu, Ping-Chiang
Citation:

Citation: Lin, Ku-Feng; Lee, Tian-Ren; Tsai, Ping-Hsing; Hsu, Ming-Pin; Chen, Ching-San; Lyu, Ping-Chiang. "Structure-based protein engineering for alpha-amylase inhibitory activity of plant defensin."  Proteins 68, 530-540 (2007).
PubMed: 17444520

Assembly members:

Assembly members:
VrD2, polymer, 47 residues, 5495 Da.

Natural source:

Natural source:   Common Name: Mung bean   Taxonomy ID: 157791   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Vigna radiata

Experimental source:

Experimental source:   Production method: Purified from the natural source

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts288

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VrD21

Entities:

Entity 1, VrD2 47 residues - 5495 Da.

1   LYSTHRCYSGLUASNLEUALAASNTHRTYR
2   ARGGLYPROCYSPHETHRTHRGLYSERCYS
3   ASPASPHISCYSLYSASNLYSGLUHISLEU
4   ARGSERGLYARGCYSARGASPASPPHEARG
5   CYSTRPCYSTHRARGASNCYS

Samples:

sample_1: VrD2 2 ± 0.1 mM; Phosphate buffer 50 ± 1 mM; D2O 10%; H2O 90%

conditions_1: ionic strength: 50 mM; pH: 6.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H_2DNOESYsample_1not availableconditions_1
1H_2DTOCSYsample_1not availableconditions_1
1H_2DDQFCOSYsample_1not availableconditions_1

Software:

SPARKY v3.111 -

NMR spectrometers:

  • Bruker avance 600 MHz

Related Database Links:

PDB
DBJ BAB82453