BMRB Entry 7209

Title:
Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis.
Deposition date:
2006-07-04
Original release date:
2006-10-02
Authors:
Golovanov, Alexander; Balasingham, Seetha; Tzitzilonis, Christos; Goult, Benjamin; Lian, Lu-Yun; Homberset, Havard; Tonjum, Tone; Derrick, Jeremy
Citation:

Citation: Golovanov, Alexander; Balasingham, Seetha; Tzitzilonis, Christos; Goult, Benjamin; Lian, Lu-Yun; Homberset, Havard; Tonjum, Tone; Derrick, Jeremy. "Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis"  J. Biomol. NMR 36, 68-68 (2006).
PubMed: 16933020

Assembly members:

Assembly members:
PilP, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET101/D-TOPO

Data sets:
Data typeCount
13C chemical shifts436
15N chemical shifts116
1H chemical shifts749

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PilP1

Entities:

Entity 1, PilP 113 residues - Formula weight is not available

1   GLUTHRASPLYSLYSGLYGLUASNALAPRO
2   ASPTHRLYSARGILELYSGLUTHRLEUGLU
3   LYSPHESERLEUGLUASNMETARGTYRVAL
4   GLYILELEULYSSERGLYGLNLYSVALSER
5   GLYPHEILEGLUALAGLUGLYTYRVALTYR
6   THRVALGLYVALGLYASNTYRLEUGLYGLN
7   ASNTYRGLYARGILEGLUSERILETHRASP
8   ASPSERILEVALLEUASNGLULEUILEGLU
9   ASPSERTHRGLYASNTRPVALSERARGLYS
10   ALAGLULEULEULEUASNSERSERASPLYS
11   ASNTHRGLUGLNALAALAALAPROALAALA
12   GLUGLNASN

Samples:

sample_1: PilP, [U-13C; U-15N], 0.5 – 1.0 mM; phosphate buffer 20 mM; EDTA 1 mM; H2O 95%; D2O 5%

sample_2: PilP0.5 – 1.0 mM; phosphate buffer 20 mM; EDTA 1 mM; H2O 95%; D2O 5%

conditions_1: pH: 6.1; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availableconditions_1
HNCACOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCOCAnot availablenot availableconditions_1
HBHACONHnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
NOESY-HSQCnot availablenot availableconditions_1
TOCSY-HSQCnot availablenot availableconditions_1
NOESYnot availablenot availableconditions_1
TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE DRX 600 MHz

Related Database Links:

PDB
EMBL CAM07914 CAM09717 CAX50761 CBA04083 CBA04812
GB AAB52532 AAF42147 AAW88856 ABX72622 ACF28836
REF NP_274808 WP_002214522 WP_002217909 WP_002219788 WP_002224058

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks