BMRB Entry 7297

Title:
Assignment of conserved hypothetical protein RPA1320 from Rhodopseudomonas Palustris; Northeast Structural Genomics Consortium Target RPT3 / Ontario Center for Structural Proteomics Target RP1313
Deposition date:
2006-09-14
Original release date:
2006-10-23
Authors:
Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Karra, Murthy; Gutmanas, Aleksandras; Guido, Valeria; Arrowsmith, Cheryl
Citation:

Citation: Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Karra, Murthy; Gutmanas, Aleksandras; Guido, Valeria; Arrowsmith, Cheryl. "Solution structure of RPA1320"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
conserved hypotetical protein rpa1320, polymer, 102 residues, 11412 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Rhodopseudomonas palustris   Taxonomy ID: 258594   Superkingdom: Bacteria   Kingdom: not available   Genus/species: rhodopseudomonas palustris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: P11

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts95
1H chemical shifts648

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rpa13201
2ZINC ion, 12
3ZINC ion, 22

Entities:

Entity 1, rpa1320 102 residues - 11412 Da.

1   METTHRMETGLYCYSARGHISVALALAGLY
2   ILEARGTHRVALTHRPROSERALALEUGLY
3   CYSGLUGLUCYSLEULYSILEGLYSERPRO
4   TRPVALHISLEUARGILECYSARGTHRCYS
5   GLYHISVALGLYCYSCYSASPASPSERPRO
6   HISLYSHISALATHRARGHISPHEHISALA
7   THRGLYHISPROILEILEGLUGLYTYRASP
8   PROPROGLUGLYTRPGLYTRPCYSTYRVAL
9   ASPGLUVALMETPHEASPLEUSERASPARG
10   METTHRPROHISASNGLYPROILEPROARG
11   TYRVAL

Entity 2, ZINC ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: conserved hypotetical protein rpa1320, [U-13C; U-15N], 0.6 mM; NaCl 250 mM; TRIS 10 mM

cond_set1: ionic strength: 0.25 M; pH: 7.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCsample_1not availablecond_set1
HNCOsample_1not availablecond_set1
4D HBHACBCAcoNHsample_1not availablecond_set1
4D HCCcoNH TOCSYsample_1not availablecond_set1
13C HSQCsample_1not availablecond_set1
HC(C)H TOCSYsample_1not availablecond_set1
HCC(H) TOCSYsample_1not availablecond_set1
15N NOESYsample_1not availablecond_set1
13C NOESYsample_1not availablecond_set1

Software:

SPARKY v3.95 - spectral analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks