BMRB Entry 11146

Title:
Solution structure of the eIF-5_eIF-2B domain from human Eukaryotic translation initiation factor 5
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the eIF-5_eIF-2B domain from human Eukaryotic translation initiation factor 5"  .

Assembly members:

Assembly members:
eIF-5_eIF-2B domain, polymer, 157 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060411-05

Data sets:
Data typeCount
13C chemical shifts677
15N chemical shifts159
1H chemical shifts1079

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF-5_eIF-2B domain1
2ZINC ION2

Entities:

Entity 1, eIF-5_eIF-2B domain 157 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETSERVAL
2   ASNVALASNARGSERVALSERASPGLNPHE
3   TYRARGTYRLYSMETPROARGLEUILEALA
4   LYSVALGLUGLYLYSGLYASNGLYILELYS
5   THRVALILEVALASNMETVALASPVALALA
6   LYSALALEUASNARGPROPROTHRTYRPRO
7   THRLYSTYRPHEGLYCYSGLULEUGLYALA
8   GLNTHRGLNPHEASPVALLYSASNASPARG
9   TYRILEVALASNGLYSERHISGLUALAASN
10   LYSLEUGLNASPMETLEUASPGLYPHEILE
11   LYSLYSPHEVALLEUCYSPROGLUCYSGLU
12   ASNPROGLUTHRASPLEUHISVALASNPRO
13   LYSLYSGLNTHRILEGLYASNSERCYSLYS
14   ALACYSGLYTYRARGGLYMETLEUASPTHR
15   HISHISLYSLEUCYSTHRPHEILELEULYS
16   ASNPROPROGLUASNSERASP

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: eIF-5_eIF-2B domain, [U-13C; U-15N], 1.32 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAB15593 BAC36325 BAE34918 BAE36460 BAE37319
EMBL CAB45711 CAD97610 CAG32503 CAG32993 CAH93306
GB AAA41112 AAC50572 AAH07728 AAH15630 AAH19769
REF NP_001006488 NP_001008209 NP_001126945 NP_001180152 NP_001960
SP P55010 P59325 Q07205 Q5R4L0
TPG DAA17435
AlphaFold Q5R4L0 Q07205 P59325 P55010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks